• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

铜绿假单胞菌多鞭毛突变体的分离与鉴定

Isolation and characterization of multiflagellate mutants of Pseudomonas aeruginosa.

作者信息

Suzuki T, Iino T

出版信息

J Bacteriol. 1980 Sep;143(3):1471-9. doi: 10.1128/jb.143.3.1471-1479.1980.

DOI:10.1128/jb.143.3.1471-1479.1980
PMID:6773930
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC294538/
Abstract

Multitrichously polar flagellated mutants were isolated from a monotrichously flagellated strain of Pseudomonas aeruginosa. The ability of the mutant cells to swarm in semisolid media at given gel strengths was increased by the multiflagellation. Observations of the mutant cells by electron microscopy revealed that the number of flagella produced per cell cycle was increased. F116 phage-mediated transduction showed that the multiflagellation occurred by a single mutation and that the mutation sites were linked to a fla cluster of this organism.

摘要

从铜绿假单胞菌的单鞭毛菌株中分离出多鞭毛极性突变体。多鞭毛增加了突变体细胞在给定凝胶强度的半固体培养基中群游的能力。通过电子显微镜对突变体细胞的观察表明，每个细胞周期产生的鞭毛数量增加。F116噬菌体介导的转导表明，多鞭毛是由单个突变引起的，且突变位点与该生物体的一个鞭毛基因簇相关联。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76be/294538/f4001691617d/jbacter00570-0381-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76be/294538/33125472f0d2/jbacter00570-0379-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76be/294538/f4001691617d/jbacter00570-0381-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76be/294538/33125472f0d2/jbacter00570-0379-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76be/294538/f4001691617d/jbacter00570-0381-a.jpg

相似文献

1
Isolation and characterization of multiflagellate mutants of Pseudomonas aeruginosa.铜绿假单胞菌多鞭毛突变体的分离与鉴定
J Bacteriol. 1980 Sep;143(3):1471-9. doi: 10.1128/jb.143.3.1471-1479.1980.
2
Selection of non-flagellated and non-piliated mutants from Pseudomonas aeruginosa strain TPB-1.
Kitasato Arch Exp Med. 1987 Sep;60(3):79-86.
3
Ordering of the flagellar genes in Pseudomonas aeruginosa by insertions of mercury transposon Tn501.通过汞转座子Tn501插入对铜绿假单胞菌鞭毛基因进行排序。
J Bacteriol. 1983 Feb;153(2):1008-17. doi: 10.1128/jb.153.2.1008-1017.1983.
4
Transductional analysis of the flagellar genes in Pseudomonas aeruginosa.铜绿假单胞菌鞭毛基因的转导分析。
J Bacteriol. 1983 Feb;153(2):1018-26. doi: 10.1128/jb.153.2.1018-1026.1983.
5
[Structural elements of Pseudomonas aeruginosa based on electron microscopy findings].
Zh Mikrobiol Epidemiol Immunobiol. 1981 Jul(7):49-53.
6
Loss of virulence associated with absence of flagellum in an isogenic mutant of Pseudomonas aeruginosa in the burned-mouse model.在烧伤小鼠模型中，铜绿假单胞菌同基因突变体中与鞭毛缺失相关的毒力丧失。
Infect Immun. 1982 Dec;38(3):1296-8. doi: 10.1128/iai.38.3.1296-1298.1982.
7
Swarming of Pseudomonas aeruginosa is dependent on cell-to-cell signaling and requires flagella and pili.铜绿假单胞菌的群体游动依赖于细胞间信号传导，并且需要鞭毛和菌毛。
J Bacteriol. 2000 Nov;182(21):5990-6. doi: 10.1128/JB.182.21.5990-5996.2000.
8
Appearance of straight flagellar filaments in the presence of p-fluorophenylalanine in Pseudomonas aeruginosa.在铜绿假单胞菌中，对氟苯丙氨酸存在时直鞭毛丝的出现。
J Bacteriol. 1977 Jan;129(1):527-9. doi: 10.1128/jb.129.1.527-529.1977.
9
[Isolation, depolymerization and repolymerization of flagella of Pseudomonas aeruginosa (author's transl)].铜绿假单胞菌鞭毛的分离、解聚和再聚合（作者译）
Zentralbl Bakteriol A. 1980 Mar;246(3):363-72.
10
Flagellation of Pseudomonas aeruginosa during the cell division cycle.铜绿假单胞菌在细胞分裂周期中的鞭毛形成
Microbiol Immunol. 1982;26(2):113-7. doi: 10.1111/j.1348-0421.1982.tb00160.x.

引用本文的文献

1
Swarming of : Through the lens of biophysics.关于……的群体行为：透过生物物理学的视角
Biophys Rev (Melville). 2023 Sep;4(3):031305. doi: 10.1063/5.0128140. Epub 2023 Sep 28.
2
The Diguanylate Cyclase HsbD Intersects with the HptB Regulatory Cascade to Control Pseudomonas aeruginosa Biofilm and Motility.双鸟苷酸环化酶HsbD与HptB调控级联相互作用以控制铜绿假单胞菌的生物膜形成和运动性。
PLoS Genet. 2016 Oct 28;12(10):e1006354. doi: 10.1371/journal.pgen.1006354. eCollection 2016 Oct.
3
c-di-GMP heterogeneity is generated by the chemotaxis machinery to regulate flagellar motility.

本文引用的文献

1
Pseudomonas genetics.假单胞菌遗传学
Annu Rev Genet. 1971;5:425-46. doi: 10.1146/annurev.ge.05.120171.002233.
2
An environmentally-induced transition from the flagellated to the non-flagellated state in Salmonella typhimurium: the fate of parental flagella at cell division.鼠伤寒沙门氏菌中由环境诱导的从鞭毛状态到无鞭毛状态的转变：细胞分裂时亲代鞭毛的命运。
J Gen Microbiol. 1962 Jun;28:257-70. doi: 10.1099/00221287-28-2-257.
3
ABSENCE OF CLUSTERING OF FUNCTIONALLY RELATED GENES IN PSEUDOMONAS AERUGINOSA.铜绿假单胞菌中功能相关基因无聚类现象。
环二鸟苷酸（c-di-GMP）的异质性由趋化机制产生，以调节鞭毛运动。
Elife. 2013 Dec 17;2:e01402. doi: 10.7554/eLife.01402.
4
Flagella and pili-mediated near-surface single-cell motility mechanisms in P. aeruginosa.铜绿假单胞菌中鞭毛和菌毛介导的近表面单细胞运动机制。
Biophys J. 2011 Apr 6;100(7):1608-16. doi: 10.1016/j.bpj.2011.02.020.
5
Inorganic polyphosphate is needed for swimming, swarming, and twitching motilities of Pseudomonas aeruginosa.铜绿假单胞菌的游动、群体迁移和颤动运动需要无机多聚磷酸盐。
Proc Natl Acad Sci U S A. 2000 Apr 25;97(9):4885-90. doi: 10.1073/pnas.060030097.
6
fleN, a gene that regulates flagellar number in Pseudomonas aeruginosa.fleN，一种调节铜绿假单胞菌鞭毛数量的基因。
J Bacteriol. 2000 Jan;182(2):357-64. doi: 10.1128/JB.182.2.357-364.2000.
7
Analysis of flagellar genes in Pseudomonas aeruginosa by use of Rfla plasmids and conjugations.利用Rfla质粒和接合作用分析铜绿假单胞菌中的鞭毛基因。
J Bacteriol. 1981 Sep;147(3):1008-14. doi: 10.1128/jb.147.3.1008-1014.1981.
8
Transductional analysis of the flagellar genes in Pseudomonas aeruginosa.铜绿假单胞菌鞭毛基因的转导分析。
J Bacteriol. 1983 Feb;153(2):1018-26. doi: 10.1128/jb.153.2.1018-1026.1983.
9
Ordering of the flagellar genes in Pseudomonas aeruginosa by insertions of mercury transposon Tn501.通过汞转座子Tn501插入对铜绿假单胞菌鞭毛基因进行排序。
J Bacteriol. 1983 Feb;153(2):1008-17. doi: 10.1128/jb.153.2.1008-1017.1983.
Genet Res. 1965 Jul;6:284-99. doi: 10.1017/s0016672300004158.
4
GROWTH CHARACTERISITICS OF SOME GRAM-NEGATIVE BACTERIA.一些革兰氏阴性菌的生长特性
J Gen Microbiol. 1965 Apr;39:109-23. doi: 10.1099/00221287-39-1-109.
5
ON THE DISTINCTION BETWEEN POLAR MONOTRICHOUS AND LOPHOTRICHOUS FLAGELLATION IN GREEN FLUORESCENT PSEUDOMONADS.
Acta Pathol Microbiol Scand. 1964;60:588-98. doi: 10.1111/apm.1964.60.4.588.
6
Effects of radiation on transduction on Pseudomonas aeruginosa.辐射对铜绿假单胞菌转导的影响。
Virology. 1962 Sep;18:89-94. doi: 10.1016/0042-6822(62)90180-0.
7
Fine structure and isolation of the hook-basal body complex of flagella from Escherichia coli and Bacillus subtilis.大肠杆菌和枯草芽孢杆菌鞭毛钩-基体复合体的精细结构与分离
J Bacteriol. 1971 Jan;105(1):384-95. doi: 10.1128/jb.105.1.384-395.1971.
8
Purification and thermal stability of intact Bacillus subtilis flagella.完整枯草芽孢杆菌鞭毛的纯化及热稳定性
J Bacteriol. 1971 Jan;105(1):369-75. doi: 10.1128/jb.105.1.369-375.1971.
9
Synthesis and structure of Caulobacter crescentus flagella.新月柄杆菌鞭毛的合成与结构
J Bacteriol. 1973 Jan;113(1):478-85. doi: 10.1128/jb.113.1.478-485.1973.
10
Genetic analysis of phenylalanine-responding mutants of Pseudomonas aeruginosa.铜绿假单胞菌苯丙氨酸反应突变体的遗传分析。
J Bacteriol. 1972 Dec;112(3):1070-5. doi: 10.1128/jb.112.3.1070-1075.1972.