Sebunya T N, Saunders J R, Osborne A D
Can J Comp Med. 1983 Jan;47(1):48-53.
One group of six pigs and another group of three pigs were separately exposed in a polyethylene enclosed chamber for ten minutes, respectively, to Haemophilus pleuropneumoniae serotype 1 and Bacillus subtilis aerosols generated by an ultrasonic nebulizer. Haemophilus pleuropneumoniae and B. subtilis were deposited throughout the lungs immediately following aerosol exposure. The number of H. pleuropneumoniae and B. subtilis deposited varied within and between lungs in each group. The mean numbers of both organisms deposited in the posterior (caudal and accessory) lobes were significantly greater than those in the anterior (cranial and middle) lobes (P less than 0.001). The four principals that received H. pleuropneumoniae aerosols and the two contact controls developed fatal fibrinous pneumonia which simulated that seen in natural infections. Since this exposure system consistently resulted in clinical disease it has good potential as a model for the study of pathogenesis of the disease and more specifically for the evaluation of vaccines.
一组六头猪和另一组三头猪分别在一个用聚乙烯封闭的舱室内暴露十分钟，分别接触由超声雾化器产生的1型胸膜肺炎放线杆菌和气溶胶化的枯草芽孢杆菌。气溶胶暴露后，胸膜肺炎放线杆菌和枯草芽孢杆菌立即沉积在整个肺部。每组猪肺内和肺间胸膜肺炎放线杆菌和枯草芽孢杆菌的沉积数量各不相同。沉积在后部（尾叶和副叶）的两种细菌的平均数量显著高于前部（颅叶和中叶）（P<0.001）。接受胸膜肺炎放线杆菌气溶胶的四只实验猪和两只接触对照组猪发生了致命的纤维素性肺炎，与自然感染中所见的肺炎相似。由于这种暴露系统始终会导致临床疾病，因此作为该疾病发病机制研究模型，尤其是疫苗评估模型具有很大潜力。
