绵羊肺泡巨噬细胞的吞噬作用：在鲁米诺存在的情况下调理素浓度与发光之间的关系。
Phagocytosis by sheep alveolar macrophages: relationship between opsonin concentration and light emission in the presence of luminol.
作者信息
Ziprin R L
出版信息
Infect Immun. 1978 Mar;19(3):889-92. doi: 10.1128/iai.19.3.889-892.1978.
Abstract
The observations reported may be applied to determining the effects of various compounds, e.g., environmental pollutants and agricultural chemicals, upon the phagocytic activity of alveolar macrophages, and the method described will aid in detecting compounds which alter Fc receptor activity. A direct linear relationship existed between the concentration of antibody used to opsonize bacterial particles and the quantity of luminol-dependent light emitted by a population of sheep alveolar macrophages exposed to the opsonized particles. The relationship can be illustrated with a Lineweaver-Burk-style double-reciprocal plot. An analogy is suggested between the kinetics of enzyme substrate reactions and the interaction of antibody-coated particles with Fc receptors on cell membranes.
摘要
所报道的观察结果可用于确定各种化合物，如环境污染物和农用化学品，对肺泡巨噬细胞吞噬活性的影响，并且所描述的方法将有助于检测改变Fc受体活性的化合物。用于调理细菌颗粒的抗体浓度与暴露于调理颗粒的一群绵羊肺泡巨噬细胞发出的鲁米诺依赖性光量之间存在直接线性关系。这种关系可用Lineweaver-Burk式双倒数图来说明。有人提出酶底物反应动力学与抗体包被颗粒与细胞膜上Fc受体的相互作用之间存在类比关系。
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