Grebner J V, Mills E L, Gray G H, Quie P G
J Lab Clin Med. 1977 Jan;89(1):153-9.
The phagocytic activity and the chemiluminescence response of human polymorphonuclear neutrophils were studied with normal human serum used as opsonin for S. typhimurium and S. aureus. When serum was heated, chelated with ethyleneglycol tetraacetic acid (EGTA), or absorbed with zymosan, there were reduction of phagocytic activity for S. aureus and a comparable reduction of chemiluminescence; a 50 per cent decrease in phagocytosis by neutrophils corresponded to a 50 per cent reduction in the chemiluminescence response. In contrast, S. typhimurium was successfully phagocytized in serum treated with MgEGTA (complement--alternate pathway intact; classic pathway blocked), and chemiluminescence was reduced by only 20 per cent under the same conditions. Both neutrophil phagocytic and chemiluminescence responses for S. typhimurium were abolished when serum was heated at 56 degrees C. or adsorbed with zymosan. These findings suggest that both phagocytosis and chemiluminescence in neutrophils are interrelated, and the chemiluminescence response of neutrophils may be a reliable assay for measuring serum opsonic activity.
以正常人血清作为鼠伤寒沙门氏菌和金黄色葡萄球菌的调理素，研究了人多形核中性粒细胞的吞噬活性和化学发光反应。当血清加热、与乙二醇四乙酸（EGTA）螯合或用酵母聚糖吸附后，对金黄色葡萄球菌的吞噬活性降低，化学发光也有相应降低；中性粒细胞吞噬作用降低50%，化学发光反应也降低50%。相比之下，鼠伤寒沙门氏菌在用MgEGTA处理的血清中（补体替代途径完整；经典途径阻断）能成功被吞噬，在相同条件下化学发光仅降低20%。当血清在56℃加热或用酵母聚糖吸附时，对鼠伤寒沙门氏菌的中性粒细胞吞噬和化学发光反应均被消除。这些发现表明，中性粒细胞的吞噬作用和化学发光相互关联，中性粒细胞的化学发光反应可能是检测血清调理活性的可靠方法。
