Mayer P, Walzl H
Infection. 1983 Mar-Apr;11(2):87-96. doi: 10.1007/BF01641073.
An experimental Pseudomonas aeruginosa lung infection was induced by aerosol exposure in mice which had been pretreated with cyclophosphamide. A single dose of 200 mg/kg cyclophosphamide resulted in leukopenia which lasted for three days. At the lowest PMN levels, the mice were exposed to aerosols containing varying doses of bacteria. The survival time of the mice and the number of viable bacteria in their lungs were determined. A dramatic rise in the viable counts of Pseudomonas organisms was found between 18 and 24 hours after infection. Mice which had not been pretreated with cyclophosphamide remained healthy and did not show any lung lesions. The number and phagocytic function of the alveolar macrophages remained unaltered after cyclophosphamide treatment. Thus, PMNs seem to play an important role in the lung's early defence mechanisms against Pseudomonas aeruginosa. This animal model could be of use in evaluating additional therapies for lung infections caused by Pseudomonas aeruginosa.
通过对经环磷酰胺预处理的小鼠进行气溶胶暴露，诱导其发生实验性铜绿假单胞菌肺部感染。200mg/kg的单剂量环磷酰胺导致白细胞减少，持续三天。在中性粒细胞水平最低时，将小鼠暴露于含有不同剂量细菌的气溶胶中。测定小鼠的存活时间及其肺内活菌数量。感染后18至24小时，发现铜绿假单胞菌活菌数量急剧上升。未用环磷酰胺预处理的小鼠保持健康，未出现任何肺部病变。环磷酰胺治疗后，肺泡巨噬细胞的数量和吞噬功能保持不变。因此，中性粒细胞似乎在肺部针对铜绿假单胞菌的早期防御机制中发挥重要作用。该动物模型可用于评估治疗铜绿假单胞菌引起的肺部感染的其他疗法。
