脊椎动物血液和血浆的高分辨率氢核磁共振研究。
High resolution 1H n.m.r. studies of vertebrate blood and plasma.
作者信息
Nicholson J K, Buckingham M J, Sadler P J
出版信息
Biochem J. 1983 Jun 1;211(3):605-15. doi: 10.1042/bj2110605.
Abstract
Spin echo Fourier transform proton n.m.r. spectra of whole blood contain resonances from both erythrocytes and plasma. A large number of well-resolved signals from mobile protons of low-molecular-weight metabolites in plasma and serum have been identified. Spectra from the plasmas of eight animal species and commercial, quality control sera are compared. CaEDTA2- and MgEDTA2- resonances can be used for the simultaneous determination of EDTA-chelatable calcium and magnesium concentrations in intact plasma and other biological fluids. Cholesterol is too immobile to contribute to the spectra of intact plasma, but is readily estimated by n.m.r. in both its free and esterified forms after extraction into methanol.
摘要
全血的自旋回波傅里叶变换质子核磁共振谱包含来自红细胞和血浆的共振信号。已经鉴定出血浆和血清中低分子量代谢物的可移动质子产生的大量分辨率良好的信号。比较了八种动物物种的血浆和商业质量控制血清的光谱。CaEDTA2-和MgEDTA2-共振可用于同时测定完整血浆和其他生物流体中可被EDTA螯合的钙和镁的浓度。胆固醇流动性太差，对完整血浆的光谱没有贡献，但在萃取到甲醇中后，通过核磁共振很容易以游离和酯化形式进行估算。
相似文献
1
High resolution 1H n.m.r. studies of vertebrate blood and plasma.脊椎动物血液和血浆的高分辨率氢核磁共振研究。
Biochem J. 1983 Jun 1;211(3):605-15. doi: 10.1042/bj2110605.
2
Analysis of biological fluids using 600 MHz proton NMR spectroscopy: application of homonuclear two-dimensional J-resolved spectroscopy to urine and blood plasma for spectral simplification and assignment.使用600兆赫质子核磁共振波谱法分析生物体液：同核二维J分辨波谱法在尿液和血浆光谱简化与归属中的应用
J Pharm Biomed Anal. 1993 Jan;11(1):21-31. doi: 10.1016/0731-7085(93)80145-q.
3
[Determination of calcium, sodium, potassium and magnesium in human blood sera by atomic absorption].
Acta Pharm Hung. 1974 Nov;44(6):253-61.
4
Simple pulse-acquire NMR methods for the quantitative analysis of calcium, magnesium and sodium in human serum.
Spectrochim Acta A Mol Biomol Spectrosc. 2006 Oct;65(2):254-60. doi: 10.1016/j.saa.2005.10.039. Epub 2006 Feb 21.
5
A quantitative study of the complexation of cadmium in hemolyzed human erythrocytes by 1H NMR spectroscopy.
J Inorg Biochem. 1990 Oct;40(2):141-9. doi: 10.1016/0162-0134(90)80047-2.
6
Observation of drug metabolites and endogenous compounds in human urine by 1H nuclear magnetic resonance spectroscopy.
Xenobiotica. 1986 Jan;16(1):69-77. doi: 10.3109/00498258609043507.
7
A proton nuclear magnetic resonance study of the interaction of cadmium with human erythrocytes.
Biochim Biophys Acta. 1983 Jul 14;762(4):531-41. doi: 10.1016/0167-4889(83)90057-5.
8
High-resolution 1H-NMR spectroscopy of blood plasma for metabolic studies.用于代谢研究的血浆高分辨率1H核磁共振光谱分析。
Clin Chem. 1994 Jul;40(7 Pt 1):1245-50.
9
Molecular dynamics and information on possible sites of interaction of intramyocellular metabolites in vivo from resolved dipolar couplings in localized 1H NMR spectra.通过局部1H NMR谱中的分辨偶极耦合获得的分子动力学以及体内肌细胞内代谢物可能的相互作用位点信息。
J Magn Reson. 2004 Dec;171(2):213-24. doi: 10.1016/j.jmr.2004.08.015.
10
The determination of magnesium and calcium in serum or plasma with ethylenediaminetetra-acetic acid (EDTA).用乙二胺四乙酸（EDTA）测定血清或血浆中的镁和钙。
J Comp Pathol. 1955 Oct;65(4):285-90. doi: 10.1016/s0368-1742(55)80029-x.
引用本文的文献
1
Nuclear Magnetic Resonance Based Metabolomics-A Rising Star in Traditional Chinese Medicine Research.基于核磁共振的代谢组学——中医药研究中的一颗新星。
Pharmaceuticals (Basel). 2025 Aug 12;18(8):1186. doi: 10.3390/ph18081186.
2
Lipoprotein (Sub)Fraction Analysis on the Bruker B.I. LISA Platform.基于布鲁克 B.I. LISA 平台的脂蛋白（亚）组份分析。
Methods Mol Biol. 2025;2855:445-456. doi: 10.1007/978-1-0716-4116-3_25.
3
Metabolomics studies in common multifactorial eye disorders: a review of biomarker discovery for age-related macular degeneration, glaucoma, diabetic retinopathy and myopia.常见多因素眼病的代谢组学研究：年龄相关性黄斑变性、青光眼、糖尿病视网膜病变和近视生物标志物发现的综述
Front Mol Biosci. 2024 Aug 13;11:1403844. doi: 10.3389/fmolb.2024.1403844. eCollection 2024.
4
NMR Spectroscopy in Diagnosis and Monitoring of Methylmalonic and Propionic Acidemias.NMR 光谱学在甲基丙二酸血症和丙酸血症的诊断和监测中的应用。
Biomolecules. 2024 Apr 28;14(5):528. doi: 10.3390/biom14050528.
5
Rapid and Self-Administrable Capillary Blood Microsampling Demonstrates Statistical Equivalence with Standard Venous Collections in NMR-Based Lipoprotein Analysis.快速且可自主进行的毛细血管血微采样在基于 NMR 的脂蛋白分析中与标准静脉采集具有统计学等效性。
Anal Chem. 2024 Mar 19;96(11):4505-4513. doi: 10.1021/acs.analchem.3c05152. Epub 2024 Feb 19.
6
Longitudinal NMR-Based Metabolomics Study Reveals How Hospitalized COVID-19 Patients Recover: Evidence of Dyslipidemia and Energy Metabolism Dysregulation.基于纵向 NMR 的代谢组学研究揭示了住院 COVID-19 患者的康复情况：血脂异常和能量代谢失调的证据。
Int J Mol Sci. 2024 Jan 26;25(3):1523. doi: 10.3390/ijms25031523.
7
Performance evaluate of different chemometrics formalisms used for prostate cancer diagnosis by NMR-based metabolomics.基于 NMR 的代谢组学用于前列腺癌诊断的不同化学计量学形式的性能评估。
Metabolomics. 2023 Dec 21;20(1):8. doi: 10.1007/s11306-023-02067-x.
8
Cardiometabolic disease risk markers are increased following burn injury in children.儿童烧伤后，心血管代谢疾病风险标志物增加。
Front Public Health. 2023 Jun 2;11:1105163. doi: 10.3389/fpubh.2023.1105163. eCollection 2023.
9
Biomarker Discovery in Atherosclerotic Diseases Using Quantitative Nuclear Magnetic Resonance Metabolomics.使用定量核磁共振代谢组学发现动脉粥样硬化疾病中的生物标志物
Front Cardiovasc Med. 2021 Jul 28;8:681444. doi: 10.3389/fcvm.2021.681444. eCollection 2021.
10
Functional Metabolomics and Chemoproteomics Approaches Reveal Novel Metabolic Targets for Anticancer Therapy.功能代谢组学和化学蛋白质组学方法揭示了癌症治疗的新代谢靶标。
Adv Exp Med Biol. 2021;1280:131-147. doi: 10.1007/978-3-030-51652-9_9.
本文引用的文献
1
Endogenous phospholipase and choline release in human erythrocytes: a study using 1H-NMR spectroscopy.人红细胞内源性磷脂酶与胆碱释放：一项利用¹H-核磁共振波谱法的研究
Biochem Biophys Res Commun. 1982 Apr 29;105(4):1280-7. doi: 10.1016/0006-291x(82)90925-1.
2
N.m.r. studies of red cells.红细胞的核磁共振研究。
Philos Trans R Soc Lond B Biol Sci. 1980 Jun 25;289(1037):395-406. doi: 10.1098/rstb.1980.0056.
3
High-resolution NMR spectra of high-density serum lipoproteins.高密度血清脂蛋白的高分辨率核磁共振光谱。
Biochim Biophys Acta. 1969 Apr 29;176(3):524-36. doi: 10.1016/0005-2760(69)90219-7.
4
Structure of human serum lipoproteins: nuclear magnetic resonance supports a micellar model.人血清脂蛋白的结构：核磁共振支持胶束模型。
Science. 1968 Nov 22;162(3856):909-11. doi: 10.1126/science.162.3856.909.
5
Application of spin-echo nuclear magnetic resonance to whole-cell systems. Membrane transport.自旋回波核磁共振在全细胞系统中的应用。膜运输。
Biochem J. 1979 Apr 15;180(1):37-44. doi: 10.1042/bj1800037.
6
Human erythrocyte metabolism studies by 1H spin echo NMR.通过氢质子自旋回波核磁共振技术对人体红细胞代谢进行的研究。
FEBS Lett. 1977 Oct 1;82(1):12-6. doi: 10.1016/0014-5793(77)80875-2.
Zotero 插件