Baron M, Levitt M, Gruen R
Neuropsychobiology. 1983;9(1):9-12. doi: 10.1159/000117927.
Platelet recovery and activity of platelet monoamine oxidase (MAO) and plasma amine oxidase (PAO) were determined using different centrifugation procedures (125 g for 15 min vs. 600 g for 2.5 min) and anticoagulants. With either centrifugation procedure, the use of ethylenediaminetetraacetate (EDTA) as anticoagulant resulted in higher platelet yields and MAO activity compared to acid-citrate-dextrose (ACD). However, PAO activity was lower with EDTA as anticoagulant than with ACD. There was a trend toward higher platelet yields and higher activity levels of MAO and PAO with the 125 g centrifugation method than with the 600 g technique regardless of the anticoagulant used. The implications for MAO studies in psychiatric research were discussed.
使用不同的离心程序（125克离心15分钟与600克离心2.5分钟）和抗凝剂，测定血小板回收率以及血小板单胺氧化酶（MAO）和血浆胺氧化酶（PAO）的活性。无论采用哪种离心程序，与使用枸橼酸-葡萄糖（ACD）作为抗凝剂相比，使用乙二胺四乙酸（EDTA）作为抗凝剂可获得更高的血小板产量和MAO活性。然而，以EDTA作为抗凝剂时，PAO活性低于以ACD作为抗凝剂时。无论使用何种抗凝剂，与600克离心技术相比，采用125克离心法时，血小板产量以及MAO和PAO的活性水平有升高趋势。文中讨论了这些结果对精神病学研究中MAO研究的意义。
