ar-Rushdi A, Nishikura K, Erikson J, Watt R, Rovera G, Croce C M
Science. 1983 Oct 28;222(4622):390-3. doi: 10.1126/science.6414084.
Burkitt lymphoma cells carrying either a rearranged or unrearranged c-myc oncogene were examined with the use of probes from the 5' exon and for the second and third exon of the oncogene. The results indicate that the normal c-myc gene on chromosome 8 and the 5' noncoding and 3' coding segments of the c-myc oncogene separated by the chromosomal translocation are under different transcriptional control in the lymphoma cells. Burkitt lymphoma cells carrying a translocated but unrearranged c-myc oncogene express normal c-myc transcripts. In contrast, lymphoma cells carrying a c-myc gene rearranged head to head with the immunoglobulin constant mu region gene express c-myc transcripts lacking the normal untranslated leader.
利用来自5'外显子以及癌基因第二和第三外显子的探针，对携带重排或未重排c-myc癌基因的伯基特淋巴瘤细胞进行了检测。结果表明，8号染色体上的正常c-myc基因以及因染色体易位而分离的c-myc癌基因的5'非编码和3'编码区段，在淋巴瘤细胞中受到不同的转录调控。携带易位但未重排的c-myc癌基因的伯基特淋巴瘤细胞表达正常的c-myc转录本。相比之下，携带与免疫球蛋白恒定μ区基因头对头重排的c-myc基因的淋巴瘤细胞表达缺乏正常非翻译前导序列的c-myc转录本。
