用于总抗惊厥药物和游离抗惊厥药物分析的血清和血浆：对酶放大免疫测定技术（EMIT）分析及超滤装置的影响

Serum and plasma for total and free anticonvulsant drug analyses: effects on EMIT assays and ultrafiltration devices.

作者信息

Godolphin W, Trepanier J, Farrell K

出版信息

Ther Drug Monit. 1983;5(3):319-23. doi: 10.1097/00007691-198309000-00013.

DOI:10.1097/00007691-198309000-00013

Abstract

The suitability of serum and plasma anticoagulated with heparin, EDTA, citrate, or oxalate was assessed for analysis of free and total phenytoin, carbamazepine, and valproic acid. The free fraction was isolated by ultrafiltration through FreeLevel devices (Syva, Palo Alto, CA). Serum, heparin, and EDTA plasma were satisfactory for both free and total phenytoin and carbamazepine. EDTA could not be used for EMIT (Syva) analysis of valproate. Citrate and, to a lesser degree, oxalate cause a significant negative interference in the concentration of these three drugs as measured both by EMIT and gas-liquid chromatography.

摘要

评估了用肝素、乙二胺四乙酸（EDTA）、柠檬酸盐或草酸盐抗凝的血清和血浆用于游离及总苯妥英、卡马西平和丙戊酸分析的适用性。游离部分通过FreeLevel装置（Syva公司，加利福尼亚州帕洛阿尔托）超滤分离。血清、肝素和EDTA血浆对于游离及总苯妥英和卡马西平分析均令人满意。EDTA不能用于丙戊酸盐的酶倍增免疫测定技术（EMIT，Syva公司）分析。柠檬酸盐以及程度稍轻的草酸盐在通过EMIT和气液色谱法测定这三种药物浓度时会引起显著的负干扰。