Fischer D G, Rubinstein M
Cell Immunol. 1983 Oct 15;81(2):426-34. doi: 10.1016/0008-8749(83)90249-6.
A low-density subpopulation of normal human peripheral blood mononuclear leukocytes obtained by percoll gradients was found to produce significant levels (up to 10,000 units/ml) of interferon in the absence of an external inducer. The interferon (IFN) was characterized as a mixture of IFN-gamma (immune) and a pH-sensitive IFN-alpha by neutralization with specific antibodies, by lability to low pH, and by cross-reactivity on bovine cells. Significant levels of IFN were obtained only at high (greater than or equal to 5 X 10(6) cells/ml) cell densities. These levels were not affected by the presence of small T lymphocytes and sharply decreased with cell dilution in a nonlinear fashion. A release of IFN in the absence of an external inducer, indicates additional physiological roles besides its involvement in pathological conditions.
通过Percoll梯度法获得的正常人外周血单个核白细胞的低密度亚群，发现在无外源性诱导剂的情况下能产生显著水平（高达10,000单位/毫升）的干扰素。通过用特异性抗体中和、对低pH的不稳定性以及在牛细胞上的交叉反应性，将该干扰素（IFN）鉴定为IFN-γ（免疫性）和pH敏感的IFN-α的混合物。仅在高细胞密度（≥5×10⁶个细胞/毫升）时获得显著水平的IFN。这些水平不受小T淋巴细胞存在的影响，并且随着细胞稀释以非线性方式急剧下降。在无外源性诱导剂的情况下释放IFN，表明其除了参与病理状况外还具有其他生理作用。
