Bassler M, Depuis W, Utz E, Just H M, Daschner F D
Eur J Clin Microbiol. 1983 Oct;2(5):439-44. doi: 10.1007/BF02013901.
The bactericidal activity of azlocillin and piperacillin was tested at concentrations of 1/4 the MIC, the MIC and four-fold the MIC agents a serum-resistant Staphylococcus aureus and a serum-resistant Pseudomonas aeruginosa strain in broth, in serum and in the presence of leukocytes. The antibacterial activity of azlocillin and piperacillin in serum against Staphylococcus aureus was slightly better than in broth (p greater than 0.05); both compounds were distinctly less active against Pseudomonas aeruginosa in serum than in broth (p less than 0.05). Both antibiotics enhanced susceptibility of Pseudomonas aeruginosa to leukocyte killing without serum (p less than 0.05), whereas leukocyte killing of Staphylococcus aureus was hardly improved even at the MIC and four-fold the MIC of both compounds. The antibacterial activity of azlocillin and piperacillin against both bacterial strains was most pronounced in the presence of leukocytes and serum. A marked bactericidal effect was achieved at 1/4 the MIC, the effect not being further significantly enhanced (p greater than 0.05) at the MIC or four-fold the MIC.
在肉汤、血清以及有白细胞存在的情况下，分别以1/4最低抑菌浓度（MIC）、MIC以及4倍MIC的浓度，对阿洛西林和哌拉西林针对一株血清抗性金黄色葡萄球菌和一株血清抗性铜绿假单胞菌的杀菌活性进行了测试。阿洛西林和哌拉西林在血清中针对金黄色葡萄球菌的抗菌活性略优于在肉汤中的活性（p＞0.05）；两种化合物在血清中针对铜绿假单胞菌的活性明显低于在肉汤中的活性（p＜0.05）。两种抗生素均可增强铜绿假单胞菌在无血清情况下对白细胞杀伤的敏感性（p＜0.05），而即便在两种化合物的MIC及4倍MIC浓度下，金黄色葡萄球菌被白细胞杀伤的情况也几乎没有改善。阿洛西林和哌拉西林针对两种菌株的抗菌活性在有白细胞和血清存在的情况下最为显著。在1/4 MIC浓度时即可产生显著的杀菌效果，在MIC或4倍MIC浓度时该效果未进一步显著增强（p＞0.05）。
