大肠杆菌lysA基因的调控模式：染色体lysA-lacZ融合体的表达
Regulatory pattern of the Escherichia coli lysA gene: expression of chromosomal lysA-lacZ fusions.
作者信息
Stragier P, Borne F, Richaud F, Richaud C, Patte J C
出版信息
J Bacteriol. 1983 Dec;156(3):1198-203. doi: 10.1128/jb.156.3.1198-1203.1983.
Abstract
The regulation of lysA which encodes the last enzyme for lysine biosynthesis in Escherichia coli, diaminopimelic acid-decarboxylase, was studied by using lysA-lacZ fusions. Our results indicate an absolute requirement for the LysR product for its activation, LysR protein present in a limiting amount which can be titrated by a multicopy plasmid carrying its target site and a negative regulatory role for the LysA protein itself which decreases lysA-lacZ expression 30-fold.
摘要
通过使用lysA - lacZ融合体，对编码大肠杆菌赖氨酸生物合成最后一种酶——二氨基庚二酸脱羧酶的lysA的调控进行了研究。我们的结果表明，其激活绝对需要LysR产物，LysR蛋白以限量存在，可被携带其靶位点的多拷贝质粒滴定，并且LysA蛋白本身具有负调控作用，可使lysA - lacZ表达降低30倍。
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Regulation of diaminopimelate decarboxylase synthesis in Escherichia coli. I. Identification of a lysR gene encoding an activator of the lysA gene.大肠杆菌中二氨基庚二酸脱羧酶合成的调控。I. 编码lysA基因激活剂的lysR基因的鉴定。
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