Shamardin V A
J Hyg Epidemiol Microbiol Immunol. 1983;27(3):265-70.
Selection of the method of loading erythrocytes with preparations of immunoglobulins (IG) should be determined by the properties of the sensitins to be used. For the production of efficient antibody diagnostics it is advisable to use amidol while in the case of highly active and concentrated sera, amidol, CrCl3 and glutaraldehyde should be employed. The application of IgG preparations considerably enhances the sensitivity of PHAR in the indication of antigens. Immunoglobulin diagnostics on the basis of IG preparations of normal sera should be produced by means of rivanol, tannin or previously heated erythrocytes. In the multiform group of erythrocyte immunoglobulin diagnostics it is necessary to distinguish antibody and non-antibody preparations. Such a classification is justified with respect to differences in the purpose and the optimum methods of production of immunoglobulin diagnostics.
用免疫球蛋白（IG）制剂加载红细胞的方法选择应取决于所用致敏素的特性。为了进行高效的抗体诊断，建议使用阿米酚，而对于高活性和浓缩血清，则应使用阿米酚、氯化铬（CrCl3）和戊二醛。IgG制剂的应用显著提高了PHA在抗原检测中的灵敏度。基于正常血清IG制剂的免疫球蛋白诊断应通过利凡诺、单宁或预先加热的红细胞来进行。在红细胞免疫球蛋白诊断的多种形式中，有必要区分抗体和非抗体制剂。考虑到免疫球蛋白诊断的目的和最佳生产方法的差异，这种分类是合理的。
