灌注大鼠骨骼肌中丙酮酸脱羧作用的测定
Estimation of pyruvate decarboxylation in perfused rat skeletal muscle.
作者信息
Schadewaldt P, Münch U, Prengel M, Staib W
出版信息
Biochem Biophys Res Commun. 1983 Oct 31;116(2):456-61. doi: 10.1016/0006-291x(83)90545-4.
By the determination of pyruvate dehydrogenase activity in tissue homogenates only limited information is gained on the actual metabolic flux. We therefore determined pyruvate decarboxylation in isolated rat hindlimbs non recirculating perfused with physiological (1-14C)pyruvate levels. On the basis of perfusate pyruvate specific activity a 14CO2 production of 15.8 +/- 0.5 nmol/min per g muscle was measured. However, by this method the actual pyruvate flux through the enzyme complex is underestimated by a factor of 7 due to the intracellular dilution of label.
通过测定组织匀浆中的丙酮酸脱氢酶活性，只能获得关于实际代谢通量的有限信息。因此，我们测定了用生理水平（1-14C）丙酮酸进行非循环灌注的离体大鼠后肢中的丙酮酸脱羧作用。根据灌注液中丙酮酸的比活性，测得每克肌肉中14CO2的产生量为15.8±0.5 nmol/分钟。然而，由于标记物在细胞内的稀释，通过这种方法测得的通过酶复合物的实际丙酮酸通量被低估了7倍。
Zotero 插件