Robinson J D, Risby D, Riley G, Aherne G W
J Pharmacol Exp Ther. 1978 May;205(2):499-502.
A sensitive radioimmunoassay for amitriptyline and nortriptyline in blood has been developed. The antibodies used in the radioimmunoassay were raised in a sheep against a conjugate of nortriptyline and bovine serum albumin. Using tritiated amitriptyline as the label, the assay is capable of detecting concentrations as low as 2.0 ng/ml in a 50 microliter sample of plasma. Cross-reactivity studies have demonstrated the specificity of the radioimmunoassay for both amitriptyline and nortriptyline, and comparison with gas-liquid chromatography assay has indicated the applicability of the assay to a routine situation. The radioimmunoassay has been used to study the plasma drug levels after single oral administration of amitriptyline to four volunteers. A wide variation in maximum drug concentrations, ranging from 18 to 62 ng/ml, was seen, with the time taken to reach the maxima ranging between 1.5 and 3 hours. A second concentration peak was seen in three of the volunteers, at 4 to 5 hours after ingestion of the drug.
已开发出一种用于检测血液中阿米替林和去甲替林的灵敏放射免疫分析法。放射免疫分析中使用的抗体是用去甲替林与牛血清白蛋白的偶联物在绵羊体内产生的。以氚标记的阿米替林作为标记物，该分析法能够在50微升血浆样本中检测低至2.0纳克/毫升的浓度。交叉反应研究证明了该放射免疫分析法对阿米替林和去甲替林的特异性，与气液色谱分析法的比较表明该分析法适用于常规情况。该放射免疫分析法已用于研究四名志愿者单次口服阿米替林后的血浆药物水平。观察到最大药物浓度有很大差异，范围为18至62纳克/毫升，达到最大值所需时间在1.5至3小时之间。三名志愿者在服药后4至5小时出现了第二个浓度峰值。
