Virtanen R
Scand J Clin Lab Invest. 1980 Apr;40(2):191-7. doi: 10.3109/00365518009093025.
A sensitive radioimmunoassay for the determination of tricyclic antidepressants is described. The antibodies were produced in rabbits immunized with a conjugate of N-succinyl-nortriptyline and human serum albumin and they were shown to be specific for the tricyclic antidepressant group of drugs. The assay was tested for the determination of amitriptyline and nortriptyline concentrations in plasma or serum. Specificity was achieved by coupling a simple extraction procedure (based on selective extraction of amitriptyline and nortriptyline at different pH-values) to the assay. Using [3H]imipramine as the tracer the sensitivity of the assay is 3.8-18.0 nmol/l depending on whether the extraction procedure is used or not and it can be carried out with 50-200 microliter sample volumes. Because of its advantages (sensitivity, specificity, simplicity, small sample volumes, low cost) this procedure is suitable for drug monitoring both during controlled tricyclic therapy and pharmacokinetic studies.
本文描述了一种用于测定三环类抗抑郁药的灵敏放射免疫分析方法。抗体是在兔体内产生的，这些兔用N-琥珀酰去甲替林与人血清白蛋白的偶联物免疫，结果表明它们对三环类抗抑郁药具有特异性。该分析方法用于测定血浆或血清中阿米替林和去甲替林的浓度。通过将一个简单的提取程序（基于在不同pH值下选择性提取阿米替林和去甲替林）与该分析方法相结合来实现特异性。使用[3H]丙咪嗪作为示踪剂，根据是否使用提取程序，该分析方法的灵敏度为3.8 - 18.0纳摩尔/升，并且可以用50 - 200微升的样品体积进行测定。由于其优点（灵敏度、特异性、简单性、小样品体积、低成本），该程序适用于三环类药物控制治疗期间的药物监测和药代动力学研究。
