Seto Shintaro, Kenri Tsuyoshi, Tomiyama Tetsuo, Miyata Makoto
Department of Biology, Graduate School of Science, Osaka City University, Sumiyoshi-ku, Osaka 558-8585, Japan.
J Bacteriol. 2005 Mar;187(5):1875-7. doi: 10.1128/JB.187.5.1875-1877.2005.
To examine the participation of P1 adhesin in gliding of Mycoplasma pneumoniae, we examined the effects of an anti-P1 monoclonal antibody on individual gliding mycoplasmas. The antibody reduced the gliding speed and removed the gliding cells from the glass over time in a concentration-dependent manner but had only a slight effect on nongliding cells, suggesting that the conformational changes of P1 adhesin and its displacement are involved in the gliding mechanism.
为了研究P1黏附素在肺炎支原体滑行中的作用,我们检测了抗P1单克隆抗体对单个滑行支原体的影响。随着时间的推移,该抗体以浓度依赖的方式降低了滑行速度,并使滑行的细胞从玻璃表面脱落,但对非滑行细胞只有轻微影响,这表明P1黏附素的构象变化及其移位参与了滑行机制。