Bell J W, Garry R F, Waite M R
J Virol. 1978 Mar;25(3):764-9. doi: 10.1128/JVI.25.3.764-769.1978.
Lowering the NaCl concentration of the medium inhibits the release of Sindbis virus from infected chicks cells at a stage after the nucleocapsids have bound to the membranes of the infected cells. The failure of trypsin treatment to release the inhibited virus and the ratio of the proteins in the inhibited cells make it seem likely that the inhibited virus is all intracellular. Experiments using antisera specific for E1 and E2, the envelope glycoproteins of Sindbis, suggest that the inhibitory effect of low-salt medium is mediated through an effect on E2. Lactoperoxidase radioiodination experiments indicate that, even when cleaved from PE2, E2 is not exposed on the surface of low-NaCl-treated chick cells.
降低培养基中氯化钠的浓度会在核衣壳与受感染细胞的膜结合后的某个阶段抑制辛德毕斯病毒从受感染的鸡细胞中释放。胰蛋白酶处理未能释放受抑制的病毒，以及受抑制细胞中蛋白质的比例表明，受抑制的病毒很可能全部位于细胞内。使用针对辛德毕斯包膜糖蛋白E1和E2的特异性抗血清进行的实验表明，低盐培养基的抑制作用是通过对E2的影响介导的。乳过氧化物酶放射性碘化实验表明，即使从PE2上裂解下来，E2也不会暴露在低氯化钠处理的鸡细胞表面。
