短小芽孢杆菌中低频、pbsi介导的质粒转导
Low-frequency, pbsi-mediated plasmid transduction in Bacillus pumilus.
作者信息
Bramucci M G, Lovett P S
出版信息
J Bacteriol. 1976 Aug;127(2):829-31. doi: 10.1128/jb.127.2.829-831.1976.
Abstract
Three bacteriophages were tested for ability to transduce the plasmid of pPL10 between W mutant derivatives of Bacillus pumilus NRS 576. Phage PBP1- and PMB1-generated plasmid transductants occurred at about 10% the frequency of transductants for a chromosome marker. Phage PBS1-generated plasmid transductants occurred at less than 0.1% the frequency of transductants for a chromosome marker. Possible reasons for the extremely reduced capacity of PBS1 to generate plasmid transductants are discussed.
摘要
测试了三种噬菌体在短小芽孢杆菌NRS 576的W突变衍生物之间转导pPL10质粒的能力。噬菌体PBP1和PMB1产生的质粒转导子出现的频率约为染色体标记转导子频率的10%。噬菌体PBS1产生的质粒转导子出现的频率不到染色体标记转导子频率的0.1%。讨论了PBS1产生质粒转导子能力极度下降的可能原因。
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