Park C H, Hixon D L, Ferguson C B, Hall S L, Risheim C C, Cook C B
Am J Clin Pathol. 1984 Mar;81(3):341-5. doi: 10.1093/ajcp/81.3.341.
Automated radiometric technic (BACTEC Johnston Laboratories, Towson, MD) was compared with conventional mycobacterial culture procedure (Lowenstein-Jensen plus Gruft modification of Lowenstein-Jensen) in this study of 1,000 clinical specimens. In addition, 8-azaguanine inhibition was tested by radiometric technic as a rapid procedure for the differentiation of Mycobacterium tuberculosis from other mycobacterial species. A total of 59 mycobacteria was recovered. Of 28 clinically significant isolates (M. tuberculosis, M. kansasii, M. avium, M. fortuitum), the BACTEC system detected 26 (93%). Conventional methods recovered 23 (82%). The BACTEC system required an average of seven days to recover M. tuberculosis from smear-positive specimens compared with 18 days required by Lowenstein-Jensen or Gruft slants. From smear-negative specimens, the BACTEC detected M. tuberculosis in an average of 20 days versus 28 days by conventional procedure. All 20 isolates of M. tuberculosis were inhibited by 8-azaguanine, whereas 39 isolates of mycobacteria other than M. tuberculosis were not inhibited. The BACTEC system accomplishes more rapid recovery of mycobacteria and provides a higher yield than conventional methods.
在这项对1000份临床标本的研究中，将自动放射技术（BACTEC约翰斯顿实验室，马里兰州陶森）与传统分枝杆菌培养方法（罗氏培养基加格鲁夫改良罗氏培养基）进行了比较。此外，通过放射技术测试了8-氮杂鸟嘌呤抑制作用，作为区分结核分枝杆菌与其他分枝杆菌种类的快速方法。总共分离出59株分枝杆菌。在28株具有临床意义的分离株（结核分枝杆菌、堪萨斯分枝杆菌、鸟分枝杆菌、偶然分枝杆菌）中，BACTEC系统检测出26株（93%）。传统方法分离出23株（82%）。对于涂片阳性标本，BACTEC系统平均需要7天来分离出结核分枝杆菌，而罗氏培养基或格鲁夫斜面培养基则需要18天。对于涂片阴性标本，BACTEC系统平均20天检测出结核分枝杆菌，而传统方法需要28天。所有20株结核分枝杆菌分离株均被8-氮杂鸟嘌呤抑制，而39株非结核分枝杆菌分离株未被抑制。BACTEC系统比传统方法能更快地分离出分枝杆菌，且分离率更高。
