Liébana E, Aranaz A, Mateos A, Vilafranca M, Gomez-Mampaso E, Tercero J C, Alemany J, Suarez G, Domingo M, Dominguez L
Departamento de Patología Animal I (Sanidad Animal), Facultad de Veterinaria, Universidad Complutense de Madrid, Spain.
J Clin Microbiol. 1995 Jan;33(1):33-6. doi: 10.1128/jcm.33.1.33-36.1995.
Mycobacterium bovis is a slowly growing microorganism, and confirmation of the diagnosis by conventional culture is a lengthy process. A simple, rapid method for the extraction of DNA from bovine tissue samples was developed and used in a PCR designed for the diagnosis of tuberculosis. Tissues from 81 cattle from tuberculosis-infected herds (group 1) and 19 cattle from tuberculosis-free herds (group 2) were tested in this PCR, and the results were compared with those of conventional culture. The PCR assay detected 71.4% of the culture-positive animals from group 1. Tissue from all animals in group 2 were negative in the PCR assay and by culture. The described method could be used as a rapid screening technique which would be complementary to culture of tissue specimens for the routine diagnosis of bovine tuberculosis. The PCR technique is much faster than culture and reduces the time for diagnosis from several months to 2 days. It also provides for the detection of M. bovis when rapidly growing Mycobacterium spp. are present in the sample and may be able to detect the presence of M. bovis in samples even when organisms have become nonviable.
牛分枝杆菌是一种生长缓慢的微生物，通过传统培养来确诊是一个漫长的过程。已开发出一种从牛组织样本中提取DNA的简单、快速方法，并将其用于旨在诊断结核病的聚合酶链反应（PCR）中。对来自结核病感染牛群的81头牛（第1组）和来自无结核病牛群的19头牛（第2组）的组织进行了该PCR检测，并将结果与传统培养结果进行比较。PCR检测法检测出第1组中71.4%的培养阳性动物。第2组所有动物的组织在PCR检测和培养中均为阴性。所描述的方法可作为一种快速筛查技术，它将补充组织标本培养，用于牛结核病的常规诊断。PCR技术比培养快得多，将诊断时间从数月缩短至2天。当样本中存在快速生长的分枝杆菌属时，它还能检测出牛分枝杆菌，甚至当生物体已无活力时，也可能检测出样本中牛分枝杆菌的存在。
