Ellner P D, Kiehn T E, Cammarata R, Hosmer M
Clinical Microbiology Service, Presbyterian Hospital, Columbia-Presbyterian Medical Center, New York, New York.
J Clin Microbiol. 1988 Jul;26(7):1349-52. doi: 10.1128/jcm.26.7.1349-1352.1988.
The combination of radiometric methodology (BACTEC 12B) and probe technology for recovery and identification of mycobacteria was studied in two large hospital laboratories. The sediment from vials with positive growth indices was tested with DNA probes specific for Mycobacterium tuberculosis, Mycobacterium avium, and Mycobacterium intracellulare. The sensitivity of the radiometric method and the specificity of the probes resulted in a marked reduction in the time to the final report. Biochemical testing could be eliminated on isolates giving a positive reaction with one of the probes. Some 176 isolates of M. tuberculosis, 110 of M. avium, and 5 of M. intracellulare were recovered. Two-thirds of these isolates were detected and identified within 2 weeks of inoculation and the remainder was detected by 4 weeks, a reduction of 5 to 7 weeks to the final report.
在两家大型医院实验室中，对用于分枝杆菌培养及鉴定的放射性测量方法（BACTEC 12B）与探针技术的联合应用进行了研究。对生长指数为阳性的小瓶中的沉淀物，使用针对结核分枝杆菌、鸟分枝杆菌和胞内分枝杆菌的DNA探针进行检测。放射性测量方法的敏感性和探针的特异性使得最终报告时间显著缩短。对于与其中一种探针呈阳性反应的分离株，可省去生化检测。共培养出约176株结核分枝杆菌、110株鸟分枝杆菌和5株胞内分枝杆菌。其中三分之二的分离株在接种后2周内被检测和鉴定出来，其余的在4周内被检测到，最终报告时间缩短了5至7周。
