Sengupta Mahuya, Sharma Gauri Dutta, Chakraborty Biswajit
Department of Biotechnology, Assam University, Silchar-788 011, Assam, India.
Asian Pac J Trop Biomed. 2011 Jun;1(3):193-9. doi: 10.1016/S2221-1691(11)60026-9.
To evaluate the hepatoprotective and immunotherapeutic effects of aqueous extract of turmeric rhizome in CCl4 intoxicated Swiss albino mice.
First group of mice (n=5) received CCl4 treatment at a dose of 0.5 mL/kg bw (i.p.) for 7 days. Second group was fed orally the aqueous extract of turmeric at a dose of 50 mg/kg bw for 15 days. The third group was given both the turmeric extract (for 15 days, orally) and CCl4 (for last 7 days, i.p.). The fourth group was kept as a control. To study the liver function, the transaminase enzymes (SGOT and SGPT) and bilirubin level were measured in the serum of respective groups. For assaying the immunotherapeutic action of Curcuma longa (C. longa), non specific host response parameters like morphological alteration, phagocytosis, nitric oxide release, myeloperoxidase release and intracellular killing capacity of peritoneal macrophages were studied from the respective groups.
The result of present study suggested that CCl4 administration increased the level of SGOT and SGPT and bilirubin level in serum. However, the aqueous extract of turmeric reduced the level of SGOT, SGPT and bilirubin in CCl4 intoxicated mice. Apart from damaging the liver system, CCl4 also reduced non specific host response parameters like morphological alteration, phagocytosis, nitric oxide release, myeloperoxidase release and intracellular killing capacity of peritoneal macrophages. Administration of aqueous extract of C. longa offered significant protection from these damaging actions of CCl4 on the non specific host response in the peritoneal macrophages of CCl4 intoxicated mice.
In conclusion, the present study suggests that C. longa has immunotherapeutic properties along with its ability to ameliorate hepatotoxicity.
评估姜黄根茎水提取物对四氯化碳中毒的瑞士白化小鼠的肝脏保护和免疫治疗作用。
第一组小鼠（n = 5）以0.5 mL/kg体重的剂量腹腔注射四氯化碳，持续7天。第二组小鼠口服姜黄水提取物，剂量为50 mg/kg体重，持续15天。第三组小鼠同时给予姜黄提取物（口服15天）和四氯化碳（最后7天腹腔注射）。第四组作为对照组。为研究肝功能，测定各实验组小鼠血清中的转氨酶（谷草转氨酶和谷丙转氨酶）和胆红素水平。为分析姜黄的免疫治疗作用，研究了各实验组小鼠的非特异性宿主反应参数，如形态学改变、吞噬作用、一氧化氮释放、髓过氧化物酶释放以及腹腔巨噬细胞的细胞内杀伤能力。
本研究结果表明，给予四氯化碳会使血清中的谷草转氨酶、谷丙转氨酶和胆红素水平升高。然而，姜黄水提取物可降低四氯化碳中毒小鼠血清中的谷草转氨酶、谷丙转氨酶和胆红素水平。除了损害肝脏系统外，四氯化碳还降低了非特异性宿主反应参数，如形态学改变、吞噬作用、一氧化氮释放、髓过氧化物酶释放以及腹腔巨噬细胞的细胞内杀伤能力。给予姜黄水提取物可显著保护四氯化碳中毒小鼠腹腔巨噬细胞的非特异性宿主反应免受四氯化碳的这些损害作用。
总之，本研究表明姜黄具有免疫治疗特性，同时具有改善肝毒性的能力。
