Hopp T P
Mol Immunol. 1984 Jan;21(1):13-6. doi: 10.1016/0161-5890(84)90084-1.
Effective immunization with short polypeptide antigens has typically only been possible when the peptide is conjugated to a large carrier substance, usually a protein. Such immunizations suffer from difficulties in producing conjugates of reliable composition, and from unwanted anti-carrier immune responses. When a chemically synthesized peptide, bearing hepatitis B virus a-determinant specificity, was conjugated to a dipalmityl-lysine moiety, a significant improvement in anti-hepatitis B surface antigen response was obtained, in comparison to the corresponding peptide-keyhole limpet hemocyanin conjugate. Dipalmityl lysyl peptide conjugates are readily made by standard Merrifield synthesis procedures, and are relatively free of byproducts that might cause unwanted immune responses. Gel filtration experiments suggest that the conjugates form large aggregates, possibly micelles, which may play a significant role in the enhancement of the anti-peptide response. These properties suggest that fatty acid conjugation may be a useful procedure for producing chemically synthesized peptide vaccines.
通常只有当短肽抗原与大的载体物质（通常是蛋白质）偶联时，才有可能实现有效的免疫接种。这种免疫接种在生产成分可靠的偶联物时存在困难，并且会产生不必要的抗载体免疫反应。当一种具有乙肝病毒α决定簇特异性的化学合成肽与二棕榈酰赖氨酸部分偶联时，与相应的肽-匙孔血蓝蛋白偶联物相比，抗乙肝表面抗原反应有了显著改善。二棕榈酰赖氨酰肽偶联物可通过标准的梅里菲尔德合成程序轻松制备，并且相对不含可能引起不必要免疫反应的副产物。凝胶过滤实验表明，偶联物形成大聚集体，可能是胶束，这可能在增强抗肽反应中起重要作用。这些特性表明，脂肪酸偶联可能是生产化学合成肽疫苗的一种有用方法。
