Malkin K
Can J Comp Med. 1984 Apr;48(2):208-10.
Heat inactivation of sheep serum samples resulted in the detection of an additional 9% reactors to Leptospira hardjo that were negative on the initial test of fresh samples. Treatment with EDTA gave results generally similar to heat inactivation suggesting that complement was responsible for the inhibition of agglutination. Tests on heat inactivated serum from experimentally infected sheep and goats revealed enhanced titers or reactions which were not detected in fresh serum.
绵羊血清样本的热灭活导致检测到另外9%对哈氏钩端螺旋体呈反应阳性的样本,这些样本在新鲜样本的初始检测中呈阴性。用乙二胺四乙酸(EDTA)处理得到的结果与热灭活大致相似,这表明补体是凝集抑制的原因。对实验感染绵羊和山羊的热灭活血清进行的检测显示,出现了新鲜血清中未检测到的效价升高或反应增强的情况。