Milligan S R, Martin L
J Reprod Fertil. 1984 May;71(1):81-7. doi: 10.1530/jrf.0.0710081.
The hydrostatic pressures generated during controlled flushing of the mouse uterus increased at implantation and under conditions of uterine closure. These pressures may be responsible for inducing tissue damage during flushing. The possibility that samples collected by flushing might be contaminated with interstitial fluid or plasma was studied using intravenously administered 51Cr-labelled EDTA and 125I-labelled human serum albumin as markers. The presence of both tracers was detected in all flushings and was greatest in flushings from uteri with luminal closure and early implantation sites. These observations raise serious doubts about the validity of the flushing technique for analysing uterine luminal constituents in mice.
在对小鼠子宫进行控制性冲洗过程中产生的流体静压在着床时以及子宫闭合的情况下会升高。这些压力可能是冲洗过程中导致组织损伤的原因。使用静脉注射的51Cr标记的乙二胺四乙酸（EDTA）和125I标记的人血清白蛋白作为标志物，研究了通过冲洗收集的样本可能被间质液或血浆污染的可能性。在所有冲洗液中均检测到了这两种示踪剂，并且在来自有腔闭合和早期着床部位子宫的冲洗液中含量最高。这些观察结果对冲洗技术用于分析小鼠子宫腔成分的有效性提出了严重质疑。
