Mathias C J, Welch M J
Semin Nucl Med. 1984 Apr;14(2):118-27. doi: 10.1016/s0001-2998(84)80025-2.
The radiolabeling of platelets has been studied for many years, both with megakaryocytes labeled in vivo and with direct platelet labels in vitro. The major aim of this work has been to evaluate platelet interactions in vivo. This has been made possible with indium-111-labeled platelets. The radionuclide is easily imaged and can be incorporated into platelets with ease. Unfortunately, the lipophilic complex used is not platelet-specific and must be exposed only to the isolated cell population for specific labeling. This requires isolation of platelets from whole blood followed by one of many variations of differential centrifugation, buffer washes, and resuspension techniques that have been reported. The major differences in these techniques are the resuspension media, the incubation time, and the ligand used. These variations are discussed with emphasis on known platelet characteristics and specific responses to these modifications.
血小板的放射性标记已经研究多年，包括体内标记巨核细胞和体外直接标记血小板。这项工作的主要目的是评估体内血小板的相互作用。使用铟-111标记的血小板使这一目的得以实现。这种放射性核素易于成像，并且可以轻松地掺入血小板中。不幸的是，所使用的亲脂性复合物并非血小板特异性的，必须仅将其暴露于分离的细胞群体以进行特异性标记。这需要从全血中分离出血小板，然后采用已报道的多种不同的差速离心、缓冲液洗涤和重悬技术之一。这些技术的主要差异在于重悬介质、孵育时间和所使用的配体。将重点讨论这些差异，并结合已知的血小板特性以及对这些修饰的特定反应进行阐述。
