Breese G R, Frye G D, McCown T J, Mueller R A
Pharmacol Biochem Behav. 1984 Jul;21(1):145-9. doi: 10.1016/0091-3057(84)90144-8.
Antagonism of ethanol-induced depression of locomotion was observed after intracisternal injection of thyrotropin releasing hormone (TRH) and bicuculline methiodide (BICM), as well as after microinjection of these drugs into the medial septum. The present investigation compared the behavioral and physiological consequence of administering TRH and BICM into the medial septum, inferior colliculus and substantia nigra to quantitate the similarities between these compounds. BICM produced a major increase in locomotor activity when injected into the medial septum and stereotypies when injected into the substantia nigra, suggesting that GABA-containing neurons have widespread influences on motor function. The wild running and seizure activity observed after BICM injection into the inferior colliculus was also consistent with this latter view. The marked increase in rectal temperature observed when BICM was injected into the medial septum may also implicate GABAergic mechanisms in temperature control at this brain site. TRH produced no such behavioral or physiological changes when administered into these three sites. Thus, this work strongly suggests that TRH does not exert a widespread action as a GABA antagonist because TRH did not produce the same changes induced by BICM. The actions of BICM and TRH to antagonize ethanol-induced depression when microinjected into the medial septum suggests that this brain area may be a critical site for the depressant action of ethanol.
在脑池内注射促甲状腺激素释放激素（TRH）和甲硫双环胞苷（BICM）后，以及将这些药物微量注射到内侧隔区后，均观察到对乙醇诱导的运动抑制的拮抗作用。本研究比较了将TRH和BICM注射到内侧隔区、下丘和黑质后的行为和生理后果，以量化这些化合物之间的相似性。BICM注射到内侧隔区时会使运动活动显著增加，注射到黑质时会产生刻板行为，这表明含γ-氨基丁酸（GABA）的神经元对运动功能有广泛影响。将BICM注射到下丘后观察到的狂奔和癫痫活动也与后一种观点一致。将BICM注射到内侧隔区时观察到的直肠温度显著升高也可能意味着该脑区的温度控制涉及GABA能机制。将TRH注射到这三个部位时未产生此类行为或生理变化。因此，这项研究强烈表明，TRH不会作为GABA拮抗剂发挥广泛作用，因为TRH不会产生由BICM诱导的相同变化。当将BICM和TRH微量注射到内侧隔区时，它们对乙醇诱导的抑制作用的拮抗作用表明，该脑区可能是乙醇抑制作用的关键部位。
