Dasgupta A, Bala S, Dutta S N
Parasite Immunol. 1984 Jul;6(4):341-8. doi: 10.1111/j.1365-3024.1984.tb00806.x.
Five hundred and twenty-nine sera obtained from people dwelling in an area endemic for bancroftian filariasis were analysed for the presence of soluble circulating antigens (SCA) of filarial origin by counter immunoelectrophoresis and 303 were found positive. It, therefore, appears that 57.3% subjects could be diagnosed by the detection of SCA irrespective of their clinical status. Of the three groups investigated, microfilaraemic, amicrofilaraemic and clinical, SCA could be demonstrated in maximum number of sera obtained from clinical cases. The parasite specificity of the SCA was determined by enzyme linked immunosorbent assay (ELISA), immunofluorescent antibody test (IFAT), counter immunoelectrophoresis (CIEP) and gel diffusion (GD) using antisera raised against Litomosoides carinii in rabbits and antigens derived from Wuchereria bancrofti microfilariae or antigens present in sera of patients with bancroftian filariasis. This anti-carinii hyperimmune serum under study did not show positive reactions with parasites other than filaria, or with the sera obtained from pre-immunized rabbits and non-endemic controls. A demonstration of a distinct precipitin band in CIEP and GD indicates that either monospecific hyperimmune sera or monoclonal antibodies could be raised for the development of a simple method for immunodiagnosis of bancroftian filariasis.
对居住在班氏丝虫病流行地区的529份血清进行分析，采用对流免疫电泳法检测丝虫来源的可溶性循环抗原（SCA），发现303份呈阳性。因此，似乎无论临床状况如何，57.3%的受试者都可通过检测SCA进行诊断。在所调查的微丝蚴血症、无微丝蚴血症和临床三组中，从临床病例获得的血清中能检测到最多数量的SCA。利用兔抗卡里尼丝虫免疫血清以及班氏吴策线虫微丝蚴来源的抗原或班氏丝虫病患者血清中的抗原，通过酶联免疫吸附测定（ELISA）、免疫荧光抗体试验（IFAT）、对流免疫电泳（CIEP）和凝胶扩散（GD）来确定SCA的寄生虫特异性。所研究的这种抗卡里尼丝虫超免疫血清对丝虫以外的寄生虫以及从预先免疫的兔和非流行地区对照获得的血清均未显示阳性反应。在CIEP和GD中出现明显的沉淀带表明，可以制备单特异性超免疫血清或单克隆抗体，以开发一种简单的班氏丝虫病免疫诊断方法。
