Brown A R, Gottlieb P D
Immunogenetics. 1984;20(4):417-32. doi: 10.1007/BF00345616.
The expression of two idiotype (id) families (5AF6 and 3C6) associated with the BALB/c p-azophenylarsonate-specific antibody response was examined in 11 mouse strains. Eight strains produced some of one or the other of these two id families with the mean percent expression in the anti-Ar responses of id(+) strains ranging from 8 to 43% for the 5AF6 and from 2 to 10% for the 3C6 idiotype. Four strains of mice (C58, AKR, PL, and RF) thought to have Lyt-3.1-linked VL repertoire differences from other mouse strains (Lyt-3.2) were tested for their capacity to contribute to 5AF6 and 3C6 id expression. The RF strain was capable of producing 5AF6 id and small amounts of 3C6 id. Tests of Lyt-3.1 congenic strains C.AKR (AKR Lyt-3.1 on a BALB/c background) and C.C58 (C58 Lyt-3.1 on a BALB/c background) showed that C.AKR could produce 5AF6 id while C.C58 could not. 3C6 id expression was present but depressed in C.C58 mice compared with the high 3C6 id expression in C.AKR. Breeding studies mating C.C58 (bearing the required Igh-Ca-linked VH genes) to other 5AF6(-) strains showed that gene complementation could result in 5AF6 expression in F1 offspring. 5AF6(-) strains capable of complementation included CBA/J, C57BL/6J, AKR/J, and PL/J. C58/J (from which C.C58 were derived) was the only tested strain that failed to complement for 5AF6 id expression. Additional matings between C58/J[5AF6(-)] and CBA/J[5AF6(-)] showed F1 offspring could produce 5AF6 id, indicating that C58/J can contribute functional VH genes necessary for 5AF6 id expression. Depressed expression of 5AF6 and 3C6 id was noted in mice where the C58/J-derived Lyt-3.1 genotype was present. The possibility that the depression of 5AF6 and 3C6 id expression derived from C58/J mice was due to regulatory influences rather than a lack of the VL structural genes is discussed.
在11个小鼠品系中检测了与BALB/c对对氨基苯胂酸特异性抗体反应相关的两个独特型（id）家族（5AF6和3C6）的表达情况。8个品系产生了这两个id家族中的一种或另一种，在抗对氨基苯胂酸反应中，id阳性品系的平均表达百分比，5AF6家族为8%至43%，3C6独特型为2%至10%。对4个被认为与其他小鼠品系（Lyt-3.2）在Lyt-3.1连锁的VL库存在差异的小鼠品系（C58、AKR、PL和RF），检测了它们产生5AF6和3C6 id表达的能力。RF品系能够产生5AF6 id和少量3C6 id。对Lyt-3.1同源品系C.AKR（BALB/c背景的AKR Lyt-3.1）和C.C58（BALB/c背景的C58 Lyt-3.1）的检测表明，C.AKR能够产生5AF6 id，而C.C58不能。与C.AKR中3C6 id的高表达相比，C.C58小鼠中存在3C6 id表达但有所降低。将C.C58（携带所需的Igh-Ca连锁VH基因）与其他5AF6阴性品系进行杂交研究表明，基因互补可导致F1代后代中出现5AF6表达。能够实现互补的5AF6阴性品系包括CBA/J、C57BL/6J、AKR/J和PL/J。C58/J（C.C58由此品系衍生而来）是唯一经检测不能实现5AF6 id表达互补的品系。C58/J[5AF6阴性]和CBA/J[5AF6阴性]之间的进一步杂交表明，F1代后代能够产生5AF6 id，这表明C58/J能够提供5AF6 id表达所需的功能性VH基因。在存在C58/J衍生的Lyt-3.1基因型的小鼠中，观察到5AF6和3C6 id表达降低。本文讨论了C58/J小鼠中5AF6和3C6 id表达降低是由于调节影响而非缺乏VL结构基因的可能性。
