D组着色性干皮病成纤维细胞缺乏一种表观Km值较低的无嘌呤DNA内切酶。
Xeroderma pigmentosum fibroblasts of the D group lack an apurinic DNA endonuclease species with a low apparent Km.
作者信息
Kuhnlein U, Lee B, Penhoet E E, Linn S
出版信息
Nucleic Acids Res. 1978 Mar;5(3):951-60. doi: 10.1093/nar/5.3.951.
Abstract
Apurinic DNA endonuclease activity from cultured human fibroblasts was resolved into two species by phosphocellulose chromatography. The species had sedimentation coefficients of 3.3 S and 2.8 S and apparent Km's for apurinic sites of 5 and 44 nM, respectively. The low Km species was absent from extracts of cell lines XP5BE, XP6BE and XP7BE of xeroderma pigmentosum complementation group D.
摘要
通过磷酸纤维素色谱法，从培养的人成纤维细胞中分离出两种无嘌呤DNA内切酶活性物质。这两种物质的沉降系数分别为3.3 S和2.8 S，对无嘌呤位点的表观Km值分别为5 nM和44 nM。色素性干皮病互补组D的细胞系XP5BE、XP6BE和XP7BE的提取物中不存在低Km值的那种物质。
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