肾综合征出血热病毒的感染性滴定：利用免疫黏附血凝反应检测病毒生长
Infectivity titration of hemorrhagic fever with renal syndrome virus: use of immune adherence hemagglutination for detection of virus growth.
作者信息
Matsuura Y, Sugiyama K, Morita C, Morikawa S, Shiga S, Komatsu T, Akao Y, Kitamura T
出版信息
J Clin Microbiol. 1984 Sep;20(3):483-5. doi: 10.1128/jcm.20.3.483-485.1984.
Abstract
Serial dilutions of hemorrhagic fever with renal syndrome viruses were inoculated into Vero-E6 cells in microplates. After 2 weeks of incubation, infected cells were disrupted by freezing and thawing, and virus antigens were detected by immune adherence hemagglutination. The infectivity titers of the virus as determined by this method were in close agreement with those obtained by the immunofluorescent antigen endpoint method. Then, a neutralization method was established. Japanese hemorrhagic fever with renal syndrome isolates, strains SR-11 and TR-352, were found to be distinct from Hantaan virus, strain 76-118, by the neutralization test.
摘要
将肾综合征出血热病毒的系列稀释液接种到微孔板中的Vero-E6细胞中。孵育2周后，通过冻融破坏感染细胞，并通过免疫黏附血凝检测病毒抗原。用该方法测定的病毒感染性滴度与通过免疫荧光抗原终点法获得的滴度密切一致。然后，建立了一种中和方法。通过中和试验发现，日本肾综合征出血热分离株SR-11和TR-352与汉坦病毒株76-118不同。
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本文引用的文献
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J Clin Microbiol. 1981 Sep;14(3):241-6. doi: 10.1128/jcm.14.3.241-246.1981.
2
Isolation of a virus related to hemorrhagic fever with renal syndrome from urban rats in a nonendemic area.在非疫区从城市大鼠中分离出一种与肾综合征出血热相关的病毒。
J Infect Dis. 1984 Mar;149(3):473. doi: 10.1093/infdis/149.3.473.
3
An immune adherence assay for discrimination between etiologic agents of hemorrhagic fever with renal syndrome.一种用于区分肾综合征出血热病原体的免疫黏附试验。
J Infect Dis. 1984 Jan;149(1):67-73. doi: 10.1093/infdis/149.1.67.
4
Isolation of virus causing hemorrhagic fever with renal syndrome (HFRS) through a cell culture system.通过细胞培养系统分离肾综合征出血热（HFRS）病毒。
Jpn J Med Sci Biol. 1983 Feb;36(1):17-25. doi: 10.7883/yoken1952.36.17.
5
[Etiological studies of epidemic hemorrhagic fever. I. Virus isolation in Apodemus agrarius from non-endemic area and its antigenic characterization].
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 1982;4(2):73-7.
6
Isolation of Hantaan virus, the etiologic agent of Korean hemorrhagic fever, from wild urban rats.从城市野生大鼠中分离出韩国出血热的病原体——汉坦病毒。
J Infect Dis. 1982 Nov;146(5):638-44. doi: 10.1093/infdis/146.5.638.
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