Chamberlain J W, Nolan J A, Gromkowski S H, Kelley K A, Eisenstadt J M, Herrup K, Janeway C A, Weissman S M
Department of Human Genetics, Yale University School of Medicine, New Haven, CT 06510.
J Immunol. 1988 Feb 15;140(4):1285-92.
We have introduced the gene encoding the heavy chain of the human MHC class I Ag HLA-B7 into transgenic mice. The gene was shown to be expressed at both the RNA and protein level. Cell surface HLA-B7 was detected on whole spleen cells by immunoprecipitation and on purified T cells by flow cytometry (FACS). Normal mice immunized with H-2-syngeneic B7-transgenic spleen cells generated CTL capable of killing transgenic cells and B7-expressing human JY cells. Anti-HLA mAb blocked the killing of JY cells. These results indicate that the human class I Ag HLA-B7 can be expressed at the surface of transgenic spleen cells in the absence of human beta 2-microglobulin, and that a significant fraction exists in a form recognizable by nontransgenic CTL as a major histocompatibility Ag unrestricted by H-2.
我们已将编码人类MHC I类抗原HLA - B7重链的基因导入转基因小鼠。该基因在RNA和蛋白质水平均有表达。通过免疫沉淀在全脾细胞上检测到细胞表面的HLA - B7，通过流式细胞术（FACS）在纯化的T细胞上检测到该抗原。用H - 2同基因B7转基因脾细胞免疫正常小鼠，可产生能够杀伤转基因细胞和表达B7的人类JY细胞的CTL。抗HLA单克隆抗体可阻断对JY细胞的杀伤。这些结果表明，在不存在人类β2 - 微球蛋白的情况下，人类I类抗原HLA - B7可在转基因脾细胞表面表达，并且相当一部分以非转基因CTL可识别的形式存在，作为不受H - 2限制的主要组织相容性抗原。
