Scott M T, Sinsheimer J E
J Pharm Sci. 1984 Aug;73(8):1171-2. doi: 10.1002/jps.2600730839.
A reverse-phase HPLC system was used for the determination of inorganic chloride liberated in vivo from two biphenyl compounds in the rat. Oral administration of [4-36Cl]chlorobiphenyl resulted in a total yield of 27.6% of the original dose excreted over 10 d in the urine and included 1.7% of the dose as inorganic chloride. For [4,4'-36Cl]dichlorobiphenyl, the radioactivity of the original dose in the urine was 39.8% after 10 d, which included 11.5% of the dose as inorganic chloride. These results appear to represent the first direct determination of dechlorination by measurement of the inorganic chloride and suggest that biodechlorination plays a greater role in the metabolism of these compounds than previously expected.
采用反相高效液相色谱系统测定大鼠体内两种联苯化合物释放出的无机氯。口服[4-³⁶Cl]氯代联苯后，在10天内尿液中排出的原剂量总量为27.6%，其中无机氯占剂量的1.7%。对于[4,4'-³⁶Cl]二氯联苯，10天后尿液中原剂量的放射性为39.8%，其中无机氯占剂量的11.5%。这些结果似乎代表了通过测量无机氯对脱氯作用的首次直接测定，并表明生物脱氯在这些化合物的代谢中所起的作用比之前预期的更大。
