肝脏微粒体中UDP-葡萄糖醛酸基转移酶催化生成胆红素单葡萄糖醛酸酯和胆红素双葡萄糖醛酸酯的放射分析。
Radioassay of UDP-glucuronyltransferase-catalysed formation of bilirubin monoglucuronides and bilirubin diglucuronide in liver microsomes.
作者信息
Vermeir M, Vanstapel F, Blanckaert N
出版信息
Biochem J. 1984 Oct 15;223(2):455-9. doi: 10.1042/bj2230455.
Abstract
A radioassay for specific determination of the rates of UDP-glucuronic acid-dependent conversion of bilirubin into the two isomeric (C-8, C-12) bilirubin monoglucuronides and bilirubin diglucuronide is described and illustrated by its application to rat liver microsomes. The method is based on measurement of the relative amounts of radiolabel in unesterified bilirubin and its mono- and di-esterified reaction products after incubation with [14C]bilirubin as substrate. This analysis is performed by the alkaline-methanolysis procedure, combined with one of two t.l.c. systems developed in order to enhance the sensitivity, accuracy and precision of the radioassay. Results for rates of total bilirubin glucuronide formation obtained with the new assay and the standard enzyme assay based on the ethyl anthranilate diazo-method were identical. However, the sensitivity of the latter technique is approx. 10-fold lower than that of the radioassay.
摘要
本文描述了一种用于特异性测定胆红素在UDP-葡萄糖醛酸依赖性作用下转化为两种异构（C-8，C-12）胆红素单葡萄糖醛酸酯和胆红素二葡萄糖醛酸酯速率的放射分析方法，并通过将其应用于大鼠肝微粒体进行了说明。该方法基于以[14C]胆红素为底物孵育后，测量未酯化胆红素及其单酯和二酯反应产物中放射性标记的相对量。这种分析通过碱性甲醇解程序进行，并结合开发的两种薄层层析系统之一，以提高放射分析的灵敏度、准确性和精密度。用新方法和基于邻氨基苯甲酸乙酯重氮法的标准酶法获得的总胆红素葡萄糖醛酸酯形成速率的结果是相同的。然而，后一种技术的灵敏度比放射分析低约10倍。
相似文献
1
Radioassay of UDP-glucuronyltransferase-catalysed formation of bilirubin monoglucuronides and bilirubin diglucuronide in liver microsomes.肝脏微粒体中UDP-葡萄糖醛酸基转移酶催化生成胆红素单葡萄糖醛酸酯和胆红素双葡萄糖醛酸酯的放射分析。
Biochem J. 1984 Oct 15;223(2):455-9. doi: 10.1042/bj2230455.
2
Bilirubin mono- and di-glucuronide formation by purified rat liver microsomal bilirubin UDP-glucuronyltransferase.纯化的大鼠肝脏微粒体胆红素UDP-葡萄糖醛酸基转移酶催化胆红素单葡萄糖醛酸酯和双葡萄糖醛酸酯的形成。
Biochem J. 1984 Oct 15;223(2):461-5. doi: 10.1042/bj2230461.
3
Bilirubin diglucuronide formation by rat liver microsomes: demonstration by affinity and thin layer chromatography of bile pigment tetrapyrroles.
Biochem Biophys Res Commun. 1983 Dec 16;117(2):406-12. doi: 10.1016/0006-291x(83)91215-9.
4
Regulation of bilirubin glucuronide synthesis in primate (Macaca fascicularis) liver. Kinetic analysis of microsomal bilirubin uridine diphosphate glucuronyltransferase.灵长类动物（食蟹猴）肝脏中胆红素葡萄糖醛酸苷合成的调节。微粒体胆红素尿苷二磷酸葡萄糖醛酸基转移酶的动力学分析。
Gastroenterology. 1986 Aug;91(2):287-96. doi: 10.1016/0016-5085(86)90559-7.
5
Bilirubin diglucuronide formation in intact rats and in isolated Gunn rat liver.完整大鼠及分离的Gunn大鼠肝脏中胆红素二葡萄糖醛酸酯的形成
J Clin Invest. 1982 Mar;69(3):595-603. doi: 10.1172/jci110486.
6
Simultaneous determination of bilirubin and its glucuronides in liver microsomes and recombinant UGT1A1 enzyme incubation systems by HPLC method and its application to bilirubin glucuronidation studies.采用高效液相色谱法同时测定肝微粒体和重组UGT1A1酶孵育体系中胆红素及其葡糖醛酸苷，并将其应用于胆红素葡糖醛酸化研究。
J Pharm Biomed Anal. 2014 Apr;92:149-59. doi: 10.1016/j.jpba.2014.01.025. Epub 2014 Jan 27.
7
Bilirubin diglucuronide synthesis by a UDP-glucuronic acid-dependent enzyme system in rat liver microsomes.大鼠肝微粒体中由UDP-葡萄糖醛酸依赖性酶系统合成胆红素二葡萄糖醛酸酯。
Proc Natl Acad Sci U S A. 1979 Apr;76(4):2037-41. doi: 10.1073/pnas.76.4.2037.
8
Microsomal UDP-glucuronyltransferase-catalyzed bilirubin diglucuronide formation in human liver.人肝脏中微粒体UDP-葡萄糖醛酸基转移酶催化胆红素二葡萄糖醛酸酯的形成。
J Hepatol. 1986;2(2):182-94. doi: 10.1016/s0168-8278(86)80077-0.
9
Bilirubin mono- and diglucuronide formation by human liver in vitro: assay by high-pressure liquid chromatography.人肝脏体外胆红素单葡萄糖醛酸酯和双葡萄糖醛酸酯的形成：高压液相色谱法测定
Hepatology. 1981 Nov-Dec;1(6):622-7. doi: 10.1002/hep.1840010610.
10
The formation and distribution of bilirubin monoglucuronide and diglucuronide in rat liver slices.大鼠肝切片中胆红素单葡萄糖醛酸酯和双葡萄糖醛酸酯的形成与分布。
Biochem J. 1979 Jun 1;179(3):473-7. doi: 10.1042/bj1790473.
引用本文的文献
1
Hybrid Nanoparticle Platform for Nanoscale Scintillation Proximity Assay.用于纳米级闪烁邻近分析的混合纳米颗粒平台
ACS Appl Nano Mater. 2019 Mar 22;2(3):1259-1266. doi: 10.1021/acsanm.8b02136. Epub 2019 Feb 20.
2
Polystyrene-Core, Silica-Shell Scintillant Nanoparticles for Low-Energy Radionuclide Quantification in Aqueous Media.聚苯乙烯-核，硅壳闪烁体纳米粒子用于水相中的低能放射性核素定量。
ACS Appl Mater Interfaces. 2018 Feb 7;10(5):4953-4960. doi: 10.1021/acsami.7b15943. Epub 2018 Jan 24.
3
Bilirubin mono- and di-glucuronide formation by purified rat liver microsomal bilirubin UDP-glucuronyltransferase.纯化的大鼠肝脏微粒体胆红素UDP-葡萄糖醛酸基转移酶催化胆红素单葡萄糖醛酸酯和双葡萄糖醛酸酯的形成。
Biochem J. 1984 Oct 15;223(2):461-5. doi: 10.1042/bj2230461.
4
Properties of membrane-bound bilirubin UDP-glucuronyltransferase in rough and smooth endoplasmic reticulum and in the nuclear envelope from rat liver.大鼠肝脏粗面内质网、滑面内质网及核膜中膜结合胆红素UDP-葡萄糖醛酸基转移酶的特性
Biochem J. 1989 May 1;259(3):659-63. doi: 10.1042/bj2590659.
5
Urobilinogen-i is a major derivative of bilirubin in bile of homozygous Gunn rats.尿胆原-i是纯合子冈恩大鼠胆汁中胆红素的主要衍生物。
Biochem J. 1990 May 15;268(1):181-5. doi: 10.1042/bj2680181.
本文引用的文献
1
The preparation of crystalline bilirubin-C14.结晶胆红素-C14的制备
J Clin Invest. 1961 Aug;40(8 Pt 1-2):1442-52. doi: 10.1172/JCI104375.
2
3
Analysis of bilirubin and bilirubin mono- and di-conjugates. Determination of their relative amounts in biological samples.胆红素及胆红素单、双结合物的分析。测定其在生物样品中的相对含量。
Biochem J. 1980 Jan 1;185(1):115-28. doi: 10.1042/bj1850115.
4
The formation of bilirubin diglucuronide by rat liver microsomal preparations.大鼠肝微粒体制剂生成胆红素二葡萄糖醛酸酯的过程。
Can J Biochem. 1980 Nov;58(11):1302-10. doi: 10.1139/o80-175.
5
Liquid-chromatographic assay and identification of mono- and diester conjugates of bilirubin in normal serum.正常血清中胆红素单酯和双酯结合物的液相色谱测定与鉴定
Clin Chem. 1983 Oct;29(10):1767-71.
6
The identification of monoconjugates of bilirubin in bile as amide derivatives.胆汁中胆红素单共轭物作为酰胺衍生物的鉴定。
Biochem J. 1971 Dec;125(3):917-9. doi: 10.1042/bj1250917.
7
Assay and properties of dititonin-activated bilirubin uridine diphosphate glucuronyltransferase from rat liver.大鼠肝脏中地衣皂苷激活的胆红素尿苷二磷酸葡萄糖醛酸基转移酶的测定及性质
Biochem J. 1972 Sep;129(3):605-18. doi: 10.1042/bj1290605.
8
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.一种利用蛋白质 - 染料结合原理对微克级蛋白质进行定量的快速灵敏方法。
Anal Biochem. 1976 May 7;72:248-54. doi: 10.1016/0003-2697(76)90527-3.
9
The isolation and further characterization of the bilirubin tetrapyrroles in bile-containing human duodenal juice and dog gall-bladder bile.含胆汁的人十二指肠液和狗胆囊胆汁中胆红素四吡咯的分离及进一步特性分析
Biochem J. 1977 Oct 1;167(1):1-8. doi: 10.1042/bj1670001.
10
Comparison in different species of biliary bilirubin-IX alpha conjugates with the activities of hepatic and renal bilirubin-IX alpha-uridine diphosphate glycosyltransferases.不同物种中胆汁胆红素-IXα共轭物与肝脏和肾脏胆红素-IXα-尿苷二磷酸糖基转移酶活性的比较。
Biochem J. 1977 Jun 15;164(3):737-46. doi: 10.1042/bj1640737.
Zotero 插件