DiNardo S, Kuner J M, Theis J, O'Farrell P H
Cell. 1985 Nov;43(1):59-69. doi: 10.1016/0092-8674(85)90012-1.
Engrailed is required to establish and maintain developmental compartments within each segment of the fly. To understand the role of the engrailed protein in this process, we have raised antibodies against engrailed and have visualized an engrailed protein in embryos by indirect immunofluorescence. The protein accumulates in the nucleus, supporting the notion that engrailed is a regulatory factor. The first pattern of expression is in alternating segments followed by expression in every segment, suggesting that engrailed may be responding to pair-rule segmentation gene products. Overall, engrailed protein levels peak in areas undergoing morphogenesis. Finally, the complex final form of the head and terminalia derive from earlier simple subdivision of these areas into developmental fields by engrailed.
“engrailed”基因对于在果蝇的每个体节内建立和维持发育区域是必需的。为了了解“engrailed”蛋白在此过程中的作用，我们制备了针对“engrailed”的抗体，并通过间接免疫荧光在胚胎中观察到了“engrailed”蛋白。该蛋白在细胞核中积累，支持了“engrailed”是一种调节因子的观点。其最初的表达模式是在交替的体节中，随后在每个体节中表达，这表明“engrailed”可能对成对规则的体节分割基因产物做出反应。总体而言，“engrailed”蛋白水平在经历形态发生的区域达到峰值。最后，头部和尾节的复杂最终形态源于这些区域早期通过“engrailed”简单地细分为发育区域。
