Markenson J A, Lockshin M D, Fuzesi L, Warburg M, Joachim C, Morgan J W
J Lab Clin Med. 1980 Jan;95(1):40-8.
Abnormal SLE mononuclear cell responses to PHA can be corrected by removal of adherent mononuclear cells. The present study demonstrates that cell-free supernatants from allogeneic adherent cell cultures inhibit lymphocyte response and that addition of indomethacin to cultures partly blocks the inhibitory effect of the resulting supernatant. Supernatants from SLE monocyte cultures suppressed allogeneic normal T cell responses by 36% (response in supernatant 33,515 +/- 3814 cpm, media control 51,947 +/- 3173 cpm) but normal monocyte culture supernatants did not suppress (48,384 +/- 4172 vs. 47,477 +/- 3221 cpm). Early (less than 24 hr) addition of indomethacin to monocyte cultures prevented elaboration of inhibitory material. In normals, indomethacin-treated supernatants were strikingly stimulatory (response 178% +/- 24 of control), whereas similarly treated supernatants of SLE monocyte cultures were not stimulatory (response 103% +/- 8 of control). The data indicate that a soluble inhibitor of lymphocyte blastogenesis is produced by SLE monocytes.
系统性红斑狼疮（SLE）单核细胞对植物血凝素（PHA）的异常反应可通过去除贴壁单核细胞得到纠正。本研究表明，同种异体贴壁细胞培养的无细胞上清液可抑制淋巴细胞反应，并且在培养物中添加吲哚美辛可部分阻断所得上清液的抑制作用。SLE单核细胞培养物的上清液使同种异体正常T细胞反应抑制了36%（上清液中的反应为33,515±3814 cpm，培养基对照为51,947±3173 cpm），但正常单核细胞培养上清液无抑制作用（48,384±4172 vs. 47,477±3221 cpm）。在单核细胞培养物中早期（少于24小时）添加吲哚美辛可阻止抑制性物质的产生。在正常人中，经吲哚美辛处理的上清液具有显著的刺激作用（反应为对照的178%±24），而SLE单核细胞培养物经类似处理的上清液则无刺激作用（反应为对照的103%±8）。数据表明，SLE单核细胞可产生一种淋巴细胞增殖的可溶性抑制剂。
