Oen K, Schroeder M L, Krzekotowska D
Clin Exp Immunol. 1985 Nov;62(2):387-96.
In vitro IgA synthesis induced by pokeweed mitogen (PWM), Staphylococcus aureus Cowan I (STA), and combinations of STA and PWM (STA/PWM) was studied in lymphocytes of IgA deficient (sIgAd) blood donors. Cultures of T-depleted (non-T) cells with autologous or allogeneic control T, irradiated T (T), or T4 cells suggested abnormalities in non-T cell fractions in most (12/22) sIgAd donors. T cell abnormalities in themselves, detectable in six donors, did not appear to account for the failure of IgA synthesis. Repeat studies in 10 donors indicated fluctuations in in vitro IgA synthesis in four. IgA synthesis induced by STA/PWM combinations was observed in only one of eight sIgAd donors. Our findings suggest that in some donors defects leading to failure to produce IgA may not be constant and support the hypothesis of a maturation arrest in IgA+ B cells in sIgAd donors.
在缺乏IgA（sIgAd）的献血者的淋巴细胞中，研究了商陆有丝分裂原（PWM）、金黄色葡萄球菌考恩I株（STA）以及STA与PWM的组合（STA/PWM）诱导的体外IgA合成。用自体或同种异体对照T细胞、经照射的T细胞（T）或T4细胞对去除T细胞的（非T）细胞进行培养，结果表明，大多数（12/22）sIgAd献血者的非T细胞部分存在异常。在6名献血者中检测到的T细胞异常本身似乎并不能解释IgA合成失败的原因。对10名献血者的重复研究表明，4人的体外IgA合成存在波动。在8名sIgAd献血者中，只有1人观察到STA/PWM组合诱导的IgA合成。我们的研究结果表明，在一些献血者中，导致无法产生IgA的缺陷可能不是恒定不变的，这支持了sIgAd献血者中IgA+B细胞成熟停滞的假说。
