de Pauw B, Theo Wagener D J, Smeulders J, Geestman E, Wessels H, Haanen C
Cancer. 1980 Feb;45(3):516-9. doi: 10.1002/1097-0142(19800201)45:3<516::aid-cncr2820450317>3.0.co;2-t.
In order to study the nature of the spontaneously stimulated cells in Hodgkin's disease, lymphocytes from 10 patients with Hodgkin's disease and 10 healthy control subjects were separated into low- and high-density fractions. Both fractions were subdivided into T and non T cell populations by means of rosette sedimentation. In Hodgkin's disease patients, non-T cells with a high density showed a significantly increased spontaneous thymidine incorporation (mean, 5677 cpm; range, 405--26490 cpm) compared to corresponding cells from control subjects (mean, 484 cpm; range, 235--850 cpm), and to unseparated high-density lymphocytes in Hodgkin's disease patients. Evidence is presented that in Hodgkin's disease, the high-density lymphocyte fraction comprises non-T cells with an apparent potency for DNA synthesis which is, however, inhibited by T cells present in the same high-density fraction.
为了研究霍奇金病中自发刺激细胞的性质，将10例霍奇金病患者和10名健康对照者的淋巴细胞分离成低密度和高密度组分。通过玫瑰花结沉降法将这两个组分再细分为T细胞群和非T细胞群。在霍奇金病患者中，高密度的非T细胞与对照者相应细胞（平均484 cpm；范围235 - 850 cpm）以及霍奇金病患者未分离的高密度淋巴细胞相比，显示出明显增加的自发胸腺嘧啶核苷掺入（平均5677 cpm；范围405 - 26490 cpm）。有证据表明，在霍奇金病中，高密度淋巴细胞组分包含具有明显DNA合成能力的非T细胞，然而，这种能力受到同一高密度组分中存在的T细胞的抑制。
