中度嗜盐细菌——反硝化副球菌的细胞内钠钾组成。

The intracellular Na+ and K+ composition of the moderately halophilic bacterium, Paracoccus halodenitrificans.

作者信息

Sadler M, McAninch M, Alico R, Hochstein L I

出版信息

Can J Microbiol. 1980 Apr;26(4):496-502. doi: 10.1139/m80-083.

Abstract

The intracellular concentrations of Na+ and K+ in exponentially growing Paracoccus halodenitrificans were independent of the NaCl concentration of the growth medium. The observed values were approximately 100 and 300 mM for Na+ and K+, respectively. In stationary phase cells, the ultimate values for Na+ depended on the NaCl concentration of the growth medium. With cells grown in the presence of 1 M NaCl, the value was about 500 mM; for cells grown in the presence of 3 M NaCl, the value was about 1.1 M. The K+ concentration in stationary phase cells was unaffected by the NaCl concentration in the growth medium. The final value was about 100 mM. Associated with these changes were changes in the ATP pool and decreases in the activities of the NADH oxidase system and the membrane-bound ATPase. It is proposed that the decrease in the activities of these enzymes may account for the ion flows observed in stationary phase cells.

摘要

在指数生长期的嗜盐反硝化副球菌中，Na⁺和K⁺的细胞内浓度与生长培养基的NaCl浓度无关。观察到的Na⁺和K⁺值分别约为100 mM和300 mM。在稳定期细胞中，Na⁺的最终值取决于生长培养基的NaCl浓度。对于在1 M NaCl存在下生长的细胞，该值约为500 mM；对于在3 M NaCl存在下生长的细胞，该值约为1.1 M。稳定期细胞中的K⁺浓度不受生长培养基中NaCl浓度的影响。最终值约为100 mM。与这些变化相关的是ATP池的变化以及NADH氧化酶系统和膜结合ATP酶活性的降低。有人提出，这些酶活性的降低可能解释了在稳定期细胞中观察到的离子流动。