Kaufman D B
Clin Exp Immunol. 1980 Mar;39(3):722-7.
Human peripheral blood lymphocytes were isolated from healthy donors and pre-incubated with two thymic factors, FTS, a new synthetic polypeptide originally isolated and purified from serum, and thymosin Fraction V. After 24 hr of pre-incubation, the cells were washed and added to an allogeneic MLR and assayed for their ability to induce a suppressor or helper response. It was found that both factots were capable of inducing both helper and suppressor T cells and that the response was unpredictable. In both instances, more than 50% of the subjects studied responded with a helper effect. The addition of Con A augmented these effects. Since both helper and suppressor T cells can be induced by the factors studied herein, one must be aware of the potential effects on T-T and T-B balances of manipulation in clinical situations such as SLE or cellular immune deficiency states.
从健康供体中分离出人类外周血淋巴细胞，并将其与两种胸腺因子进行预孵育，这两种胸腺因子分别是FTS（一种最初从血清中分离和纯化的新型合成多肽）和胸腺素组分V。预孵育24小时后，将细胞洗涤并加入异体混合淋巴细胞反应（MLR）中，检测它们诱导抑制性或辅助性反应的能力。发现这两种因子都能够诱导辅助性T细胞和抑制性T细胞，并且反应是不可预测的。在这两种情况下，超过50%的研究对象表现出辅助性效应。添加刀豆蛋白A（Con A）增强了这些效应。由于本文研究的因子能够诱导辅助性和抑制性T细胞，因此在临床情况如系统性红斑狼疮（SLE）或细胞免疫缺陷状态下，必须意识到操作对T-T和T-B平衡的潜在影响。
