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相似文献

1
A mechanism of protein localization: the signal hypothesis and bacteria.蛋白质定位机制：信号假说与细菌
J Cell Biol. 1980 Sep;86(3):701-11. doi: 10.1083/jcb.86.3.701.
2
Genetic approaches to study export of LamB to the outer membrane.研究LamB向外膜输出的遗传学方法。
Ann Microbiol (Paris). 1982 Jan;133A(1):115-22.
3
Synthesis and maturation of lambda receptor in Escherichia coli K-12: in vivo and in vitro expression of gene lamB under lac promoter control.λ受体在大肠杆菌K-12中的合成与成熟：在乳糖启动子控制下基因lamB的体内和体外表达
Proc Natl Acad Sci U S A. 1980 Mar;77(3):1491-5. doi: 10.1073/pnas.77.3.1491.
4
Mutations altering the cellular localization of the phage lambda receptor, an Escherichia coli outer membrane protein.改变噬菌体λ受体（一种大肠杆菌外膜蛋白）细胞定位的突变
Proc Natl Acad Sci U S A. 1978 Dec;75(12):5802-6. doi: 10.1073/pnas.75.12.5802.
5
Genetic studies on mechanisms of protein localization in Escherichia coli K-12.大肠杆菌K-12中蛋白质定位机制的遗传学研究。
J Supramol Struct. 1980;13(2):147-63. doi: 10.1002/jss.400130203.
6
Insertion of a MalE beta-galactosidase fusion protein into the envelope of Escherichia coli disrupts biogenesis of outer membrane proteins and processing of inner membrane proteins.将MalEβ-半乳糖苷酶融合蛋白插入大肠杆菌包膜会破坏外膜蛋白的生物合成和内膜蛋白的加工。
J Bacteriol. 1982 Oct;152(1):133-9. doi: 10.1128/jb.152.1.133-139.1982.
7
Analysis of lambda receptor and beta-lactamase synthesis and export using cloned genes in a minicell system.利用微细胞系统中的克隆基因分析λ受体及β-内酰胺酶的合成与输出
Mol Gen Genet. 1982;185(2):302-10. doi: 10.1007/BF00330802.
8
Enhanced export of beta-galactosidase fusion proteins in prlF mutants is Lon dependent.在prlF突变体中，β-半乳糖苷酶融合蛋白的增强输出是Lon依赖性的。
J Bacteriol. 1992 Sep;174(17):5661-8. doi: 10.1128/jb.174.17.5661-5668.1992.
9
Suppressor mutations that restore export of a protein with a defective signal sequence.抑制突变可恢复具有缺陷信号序列的蛋白质的输出。
Cell. 1981 Jan;23(1):79-88. doi: 10.1016/0092-8674(81)90272-5.
10
[DNA sequence encoding the signal peptide of the lambda receptor in E. coli K 12].[编码大肠杆菌K12中λ受体信号肽的DNA序列]
C R Seances Acad Sci D. 1979 Nov 26;289(14):1033-6.

引用本文的文献

1
Lyme Disease Pathogenesis.莱姆病发病机制。
Curr Issues Mol Biol. 2021;42:473-518. doi: 10.21775/cimb.042.473. Epub 2020 Dec 23.
2
Chapter 6 Protein Sorting in the Secretory Pathway.第6章 分泌途径中的蛋白质分选
Curr Top Membr Transp. 1985;24:251-294. doi: 10.1016/S0070-2161(08)60328-7. Epub 2008 May 30.
3
Blobel and Sabatini's "Beautiful Idea": Visual Representations of the Conception and Refinement of the Signal Hypothesis.布洛贝尔和萨巴蒂尼的“美妙想法”：信号假说的构想与完善的视觉呈现
J Hist Biol. 2017 Nov;50(4):797-833. doi: 10.1007/s10739-016-9462-7.
4
Secretion of the overproduced periplasmic PhoA protein into the medium and accumulation of its precursor in phoA-transformed Escherichia coli strains: involvement of outer membrane vesicles.过表达的周质 PhoA 蛋白分泌到培养基中及其前体在 phoA 转化的大肠杆菌菌株中的积累：外膜囊泡的参与。
World J Microbiol Biotechnol. 1991 May;7(3):394-406. doi: 10.1007/BF00329408.
5
Post-translational modification and processing of Escherichia coli prolipoprotein in vitro.大肠杆菌前脂蛋白的体外翻译后修饰与加工
Proc Natl Acad Sci U S A. 1982 Apr;79(7):2255-9. doi: 10.1073/pnas.79.7.2255.
6
Molecular cloning of Vibrio cholerae enterotoxin genes in Escherichia coli K-12.霍乱弧菌肠毒素基因在大肠杆菌K-12中的分子克隆
Proc Natl Acad Sci U S A. 1982 May;79(9):2976-80. doi: 10.1073/pnas.79.9.2976.
7
Mechanisms for the incorporation of proteins in membranes and organelles.蛋白质整合到膜和细胞器中的机制。
J Cell Biol. 1982 Jan;92(1):1-22. doi: 10.1083/jcb.92.1.1.
8
Lipoprotein nature of Bacillus licheniformis membrane penicillinase.地衣芽孢杆菌膜青霉素酶的脂蛋白性质
Proc Natl Acad Sci U S A. 1981 Jun;78(6):3511-5. doi: 10.1073/pnas.78.6.3511.
9
A mutation downstream from the signal peptidase cleavage site affects cleavage but not membrane insertion of phage coat protein.信号肽酶切割位点下游的突变影响切割，但不影响噬菌体外壳蛋白的膜插入。
Proc Natl Acad Sci U S A. 1981 Mar;78(3):1717-21. doi: 10.1073/pnas.78.3.1717.
10
Molecular components of the signal sequence that function in the initiation of protein export.在蛋白质输出起始过程中发挥作用的信号序列的分子成分。
J Cell Biol. 1982 Dec;95(3):689-96. doi: 10.1083/jcb.95.3.689.

本文引用的文献

1
The mechanism of protein secretion across membranes.蛋白质跨膜分泌的机制。
Nature. 1980 Jan 31;283(5746):433-8. doi: 10.1038/283433a0.
2
Mutations which alter the function of the signal sequence of the maltose binding protein of Escherichia coli.改变大肠杆菌麦芽糖结合蛋白信号序列功能的突变。
Nature. 1980 May 8;285(5760):78-81. doi: 10.1038/285078a0.
3
Labeling of proteins with beta-galactosidase by gene fusion. Identification of a cytoplasmic membrane component of the Escherichia coli maltose transport system.通过基因融合用β-半乳糖苷酶标记蛋白质。大肠杆菌麦芽糖转运系统细胞质膜成分的鉴定。
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DNA sequence encoding the NH2-terminal peptide involved in transport of lambda receptor, an Escherichia coli secretory protein.编码参与λ受体（一种大肠杆菌分泌蛋白）转运的NH2末端肽的DNA序列。
Proc Natl Acad Sci U S A. 1980 May;77(5):2621-5. doi: 10.1073/pnas.77.5.2621.
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Sequence analysis of mutations that prevent export of lambda receptor, an Escherichia coli outer membrane protein.阻止大肠杆菌外膜蛋白λ受体输出的突变的序列分析。
Nature. 1980 May 8;285(5760):82-5. doi: 10.1038/285082a0.
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Functions of lysosomes.溶酶体的功能。
Annu Rev Physiol. 1966;28:435-92. doi: 10.1146/annurev.ph.28.030166.002251.
7
Divergent operons and the genetic structure of the maltose B region in Escherichia coli K12.大肠杆菌K12中不同的操纵子与麦芽糖B区域的遗传结构
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8
Active transport of maltose in Escherichia coli K12. Involvement of a "periplasmic" maltose binding protein.大肠杆菌K12中麦芽糖的主动运输。一种“周质”麦芽糖结合蛋白的作用。
Eur J Biochem. 1974 Aug 15;47(1):139-49. doi: 10.1111/j.1432-1033.1974.tb03677.x.
9
Isolation of the bacteriophage lambda receptor from Escherichia coli.从大肠杆菌中分离噬菌体λ受体
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10
Intracellular aspects of the process of protein synthesis.蛋白质合成过程的细胞内层面
Science. 1975 Aug 1;189(4200):347-58. doi: 10.1126/science.1096303.

蛋白质定位机制：信号假说与细菌

A mechanism of protein localization: the signal hypothesis and bacteria.

作者信息

Emr S D, Hall M N, Silhavy T J

出版信息

J Cell Biol. 1980 Sep;86(3):701-11. doi: 10.1083/jcb.86.3.701.

DOI:10.1083/jcb.86.3.701
PMID:6447703
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2110675/
Abstract

We are studying the molecular mechanism of cellular protein localization. The availability of genetic techniques, such as gene fusion in Escherichia coli, has made this problem particularly amenable to study in this prokaryote. We have constructed a variety of strains in which the gene coding for an outer membrane protein is fused to the gene coding for a normally cytoplasmic enzyme, beta-galactosidase. The hybrid proteins produced by such strains retain beta-galactosidase activity; this activity serves as a simple biochemical tag for studying the localization of the outer membrane protein. In addition, we have exploited phenotypes exhibited by certain fusion strains to isolate mutants that are altered in the process of protein export. Genetic and biochemical analyses of such mutants have provided evidence that the molecular mechanism of cellular protein localization is strinkingly similar in both bacteria and animal cells.

摘要

我们正在研究细胞蛋白质定位的分子机制。诸如大肠杆菌中的基因融合等遗传技术的可用性，使得这个问题在这种原核生物中特别适合进行研究。我们构建了多种菌株，其中编码外膜蛋白的基因与编码正常位于细胞质中的酶β-半乳糖苷酶的基因融合。这些菌株产生的杂合蛋白保留了β-半乳糖苷酶活性；这种活性作为研究外膜蛋白定位的一个简单生化标记。此外，我们利用某些融合菌株表现出的表型来分离在蛋白质输出过程中发生改变的突变体。对这些突变体的遗传和生化分析提供了证据，表明细胞蛋白质定位的分子机制在细菌和动物细胞中惊人地相似。