de Jong B, Anders G J, van de Meer I H, Zijlstra J, Wierda J
J Immunogenet. 1980 Jun;7(3):261-9. doi: 10.1111/j.1744-313x.1980.tb00935.x.
Two-way and one-way mixed lymphocyte cultures (MLC) with Chinese Hamster peripheral blood lymphocytes were performed. Reduction of the culture surface and additon of 2-mercaptoethanol to the culture medium gives a marked enhancement of the reactivity of low numbers of lymphocytes in the MLC. Genetic analysis of the mixed lymphocyte reactivity (MLR) in the animals studied so far indicates that three or four different alleles are involved. However, not all phenotypes could be traced. The samples of different stocks of Chinese Hamsters in Europe and the United States did not all share the same alleles and the samples were also different as regards the relative frequency of phenotypes. For DNA inhibition in stimulating cells mitomycin C treatment is less suitable than irradiation. Skin graft survival time was longer in animals which showed no MLR than in animals with a positive MLR.
进行了中国仓鼠外周血淋巴细胞的双向和单向混合淋巴细胞培养（MLC）。减少培养面积并在培养基中添加2-巯基乙醇可显著增强MLC中少量淋巴细胞的反应性。对目前所研究动物的混合淋巴细胞反应性（MLR）进行的遗传分析表明，涉及三到四个不同的等位基因。然而，并非所有表型都能追踪到。欧洲和美国不同种群的中国仓鼠样本并非都共享相同的等位基因，而且样本在表型的相对频率方面也存在差异。对于刺激细胞中的DNA抑制，丝裂霉素C处理不如辐射合适。无MLR的动物皮肤移植存活时间比MLR阳性的动物更长。
