Zeleznick L D, Hunt K J, Sobotka A K, Valentine M D, Tippett L O, Lichtenstein L M
J Allergy Clin Immunol. 1977 Jan;59(1):2-9. doi: 10.1016/0091-6749(77)90169-5.
This double-blind study provides evidence that skin testing with dialyzed Hymenoptera venoms is a more accurate, reliable method of diagnosing hypersensitivity to the sting of honeybee, yellow jacket, yellow hornet, white-faced hornet, and wasp than is skin testing with the corresponding whole body diagnostic allergenic extract. Furthermore, the incidence of false-positives was greatly reduced by using the dialyzed Hymenoptera venom (HDV) diagnosis. In this clinical trial, most sensitive individuals had skin test reactions greater than the diluent control at concentrations of 1 mug/ml or below. Levels of venom diagnostic of 100 mug/ml appeared to produce nonspecific local irritation. Skin test with whole body diagnostic allergenic extract did not produce a consistent differentiation between sensitive and nonsensitive individuals. No untoward reactions were seen using the dialyzed Hymenoptera venom (HDV) diagnostic.
这项双盲研究表明，与使用相应的全身体诊断性变应原提取物进行皮肤试验相比，用透析后的膜翅目毒液进行皮肤试验是一种更准确、可靠的诊断对蜜蜂、黄胡蜂、大黄蜂、白脸胡蜂和黄蜂蜇伤过敏的方法。此外，使用透析后的膜翅目毒液（HDV）诊断可大大降低假阳性的发生率。在这项临床试验中，大多数敏感个体在浓度为1微克/毫升或更低时，皮肤试验反应大于稀释剂对照。100微克/毫升的毒液诊断水平似乎会产生非特异性局部刺激。用全身体诊断性变应原提取物进行皮肤试验不能在敏感个体和非敏感个体之间产生一致的区分。使用透析后的膜翅目毒液（HDV）诊断未观察到不良反应。
