小鼠迟发型超敏反应抑制细胞的表面表型。
The surface phenotype of a suppressor cell of delayed-type hypersensitivity in the mouse.
作者信息
Thompson C H, Potter T A, McKenzie I F, Parish C R
出版信息
Immunology. 1980 May;40(1):87-96.
Delayed-type hypersensitivity (DTH) to horse red blood cells was induced in cyclophosphamide-treated CBA/H mice. The DTH reaction, represented by an increase (0 x 8--1 x 0 mm) in footpad thickness 24 h after secondary challenge, could be suppressed by the adoptive transfer of 10(7) splenic lymphocytes from syngeneic mice primed with 10(9) HRBC. The surface antigenic phenotype of the suppressor cell was determined by the formation of EA, EAC, or Ig rosettes followed by depleting the rosetted populations on Isopaque-Ficoll. The suppressor cell was found to be Ig-, FcR- and CR-, although some suppression was observed with FcR+ cells. Cell depletions with cytotoxic alloantisera and rabbit complement further characterized the suppressor cell as being Thy-1+, Ly-1+, 2-, 3-, 4-, 5+, 6+, 7-, Ia- and IJ-. This cell surface phenotype if unique and differs from the Ly-1-, 2+, 3+, I-J+ suppressor cell of antibody formation and from the recently described Ly-+, 2+, 3+ feedback suppressor T cell.
用环磷酰胺处理CBA/H小鼠，诱导其对马红细胞产生迟发型超敏反应（DTH）。二次激发后24小时足垫厚度增加（0×8 - 1×0毫米）所代表的DTH反应，可通过转输来自用10⁹个马红细胞致敏的同基因小鼠的10⁷个脾淋巴细胞而受到抑制。通过形成EA、EAC或Ig玫瑰花结，随后在Isopaque - Ficoll上耗尽形成玫瑰花结的细胞群体，来确定抑制细胞的表面抗原表型。发现抑制细胞为Ig⁻、FcR⁻和CR⁻，不过FcR⁺细胞也观察到了一些抑制作用。用细胞毒性同种抗血清和兔补体进行细胞清除进一步表明抑制细胞为Thy - 1⁺、Ly - 1⁺、2⁻、3⁻、4⁻、5⁺、6⁺、7⁻、Ia⁻和I - J⁻。这种细胞表面表型是独特的，不同于抗体形成的Ly - 1⁻、2⁺、3⁺、I - J⁺抑制细胞以及最近描述的Ly⁺、2⁺、3⁺反馈抑制性T细胞。
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