Kelly R E, Ellison G W, Myers L W, Goymerac V, Larrick S B, Kelley C C
Ann Neurol. 1981 Mar;9(3):267-72. doi: 10.1002/ana.410090309.
A pokeweed mitogen-stimulated IgG synthesis assay was used to evaluate T suppressor cell function in peripheral blood lymphocytes (PBL) obtained from multiple sclerosis (MS) patients. Autochthonous cultures of fractionated and recombined MS B and T cells exhibited less suppression of IgG synthesis than similar cultures of PBL from control subjects. No difference occurred in the levels of suppression among MS patients grouped according to disease states. When allogenic cultures of MS B and normal T cells or normal B and MS T cells were compared to autochthonous cultures of control cells, no differences were observed. The results suggest that the lower levels of suppression observed in autologous MS cell cultures are not a result of T suppressor cell dysfunction alone.
采用商陆有丝分裂原刺激的IgG合成试验，评估从多发性硬化症（MS）患者获取的外周血淋巴细胞（PBL）中T抑制细胞的功能。与对照组受试者的PBL类似培养物相比，MS患者分离和重组的B细胞及T细胞的自身培养物对IgG合成的抑制作用较弱。根据疾病状态分组的MS患者之间，抑制水平没有差异。当将MS B细胞与正常T细胞或正常B细胞与MS T细胞的同种异体培养物与对照细胞的自身培养物进行比较时，未观察到差异。结果表明，在自体MS细胞培养物中观察到的较低抑制水平并非仅由T抑制细胞功能障碍所致。
