Bogoeva M V, Antokhin A I, Romanov Iu A
Biull Eksp Biol Med. 1981 Mar;91(3):343-5.
Mitosis inhibitory activity of chalone-containing preparations extracted from cells of Ehrlich's ascites carcinoma (EAC) at varying time of day (9, 13, 17, 21 1, 5 and 9 o'clock) was studied in a temporary suspension culture of EAC. The inhibitory action of the preparations depended on the time of their extraction. This may be indicative of rhythmical alterations of the production or activity of EAC chalone. The maximum activity was demonstrated by the preparations extracted at 5, 9 and 13 o'clock. The activity of the preparations, extracted at 17, 21 and 1 o'clock was considerably less. The degree of mitotic activity inhibition also depends on the dose of duration of action of the chalone-containing preparation.
研究了在一天中不同时间点（9点、13点、17点、21点、1点、5点和9点）从艾氏腹水癌细胞（EAC）中提取的含查隆制剂在EAC临时悬浮培养中的有丝分裂抑制活性。制剂的抑制作用取决于其提取时间。这可能表明EAC查隆的产生或活性存在节律性变化。5点、9点和13点提取的制剂表现出最大活性。17点、21点和1点提取的制剂活性明显较低。有丝分裂活性的抑制程度也取决于含查隆制剂的作用剂量和作用持续时间。
