Hunt J S, McGiven A R, Jackson A E, Day W A
Ren Physiol. 1980;3(1-6):368-74. doi: 10.1159/000172785.
Glomerular cultures from mice have been established and the various cells associated with them observed microscopically by phase contrast time-lapse cinematography, after histological staining and ultrastructurally with the transmission and scanning electron microscope. The most common cell types were large 80-200 microns diameter irregular polyhedral cells appearing as flattened plate-like structures morphologically, possibly of epithelial origin. Smaller 70-100 microns diameter fusiform cells possibly derived from either endothelium or mesangium were also often seen. A third cell type, small 10-20 microns diameter circular, motile and actively phagocytic was especially noticeable in cultures of hybrid New Zealand Black/New Zealand White mice but was not present in appreciable numbers in control cultures from strains without renal disease. This third cell type appeared to have Fc receptors, stained positively with non-specific esterase and by indirect immunofluorescence with specific rabbit anti-mouse macrophage serum and had the morphological appearance of a macrophage by scanning and transmission electron microscopy. It is concluded that cells of macrophage origin are associated with the immune complex glomerulonephritis in these hybrid mice.
已建立了来自小鼠的肾小球培养物，并在组织学染色后，通过相差延时摄影术在显微镜下观察了与其相关的各种细胞，还利用透射电子显微镜和扫描电子显微镜对其进行了超微结构观察。最常见的细胞类型是直径为80 - 200微米的大型不规则多面体细 胞，形态上呈扁平的板状结构，可能起源于上皮细胞。还经常可见到直径为70 - 100微米的较小的梭形细胞，可能来源于内皮细胞或系膜细胞。第三种细胞类型是直径为10 - 20微米的小型圆形细胞，具有运动性且活跃地进行吞噬作用，在新西兰黑/新西兰白杂交小鼠的培养物中尤为明显，但在无肾脏疾病品系的对照培养物中数量不多。这种第三种细胞类型似乎具有Fc受体，用非特异性酯酶染色呈阳性，并用特异性兔抗小鼠巨噬细胞血清进行间接免疫荧光染色呈阳性，通过扫描电子显微镜和透射电子显微镜观察具有巨噬细胞的形态外观。得出的结论是，巨噬细胞来源的细胞与这些杂交小鼠的免疫复合物性肾小球肾炎有关。
