Kluskens L F, Kluskens J L, Bibbo M
Am J Clin Pathol. 1984 Sep;82(3):259-66. doi: 10.1093/ajcp/82.3.259.
The relative increase in endometrial adenocarcinoma in women has increased the need for more objective criteria in the distinction of hyperplastic and neoplastic endometrium. The authors have used the ability of lectins to detect changes in surface glycoproteins to probe the differences among proliferative endometrium, endometrial adenomatous hyperplasia and adenocarcinomas. Paraffin-embedded sections of tissue obtained by Vakutage endometrial sampling technics were stained with each of 7 FITC lectin conjugates. Thirty-four specimens were examined (7 proliferative, 12 hyperplastic, and 15 adenocarcinomatous). Wheat germ agglutinin binding was detectable in all specimens with a distribution at the cell luminal border of glandular formations irrespective of diagnosis. However, adenocarcinoma cases showed distribution along the lumenal border and the cell periphery with loss of orientation of the lectin binding. Similar alterations and increased binding were noted for Concanavalin A. The WGA binding to sections was specifically inhibitable by oligosaccharides of N-acetyl-glucosamine. The results provide an objective criterion for detection of loss of cell orientation useful in the diagnosis of endometrial adenocarcinoma in tissue fragments.
女性子宫内膜腺癌发病率的相对上升，使得区分增生性和肿瘤性子宫内膜时需要更客观的标准。作者利用凝集素检测表面糖蛋白变化的能力，来探究增殖期子宫内膜、子宫内膜腺瘤样增生和腺癌之间的差异。通过Vakutage子宫内膜采样技术获取的组织石蜡包埋切片，用7种异硫氰酸荧光素（FITC）凝集素缀合物分别进行染色。共检查了34个标本（7个增殖期、12个增生期和15个腺癌）。无论诊断结果如何，在所有标本中均可检测到麦芽凝集素结合，其分布于腺管结构的细胞腔缘。然而，腺癌病例中凝集素结合沿管腔缘和细胞周边分布，且失去了方向性。刀豆球蛋白A也有类似改变且结合增加。麦芽凝集素与切片的结合可被N-乙酰葡糖胺寡糖特异性抑制。这些结果为检测细胞方向性丧失提供了客观标准，有助于诊断组织碎片中的子宫内膜腺癌。
