Memon R S, Yonezawa S, Hasui K, Sato E
Second Department of Pathology, Faculty of Medicine, Kagoshima University, Japan.
Acta Pathol Jpn. 1990 Jul;40(7):509-16. doi: 10.1111/j.1440-1827.1990.tb01593.x.
To investigate cytoplasmic alterations in cancers of the endometrium, monoclonal antibodies against H, A and B blood group substances and related lectins such as Ulex europaeus agglutinin 1 (UEA-1), Lotus tetragonolobus agglutinin (LTA), and Griffonia simplicifolia 1 (GS1-A4, -B4) were applied to 42 cases of endometrial adenocarcinoma (EAC) and 11 adenomyoses. The control specimens consisted of normal endometria with leiomyoma or adenomyosis from patients of known blood group type. The brightest and most consistent staining was obtained with GS1-A4, showing 98% positivity in EAC in contrast to 18% in normal endometria. In contrast, expression of A antigen was seen in only 24% of EAC and in 13% of normal endometria, UEA-1 binding was seen in 81% of EAC and in 10% of normal endometria, whereas H antigen was seen in 60% of EAC and 0% of normal endometria. In adenocarcinomas, staining was seen not only along the luminal border of glands but also in the cytoplasm and the lateral or basal cell membranes, whereas in the normal endometrium staining was mostly along the luminal border. Thus, a difference in the positivity and localization of glycoconjugates was observed in neoplastic and non-neoplastic endometrium.
为研究子宫内膜癌中的细胞质改变，将针对H、A和B血型物质的单克隆抗体以及相关凝集素，如欧洲荆豆凝集素1（UEA-1）、四角豆凝集素（LTA）和简单金合欢凝集素1（GS1-A4、-B4）应用于42例子宫内膜腺癌（EAC）和11例子宫腺肌病。对照标本包括来自已知血型患者的伴有平滑肌瘤或子宫腺肌病的正常子宫内膜。GS1-A4染色最亮且最一致，EAC中的阳性率为98%，而正常子宫内膜中为18%。相比之下，仅24%的EAC和13%的正常子宫内膜可见A抗原表达，81%的EAC和10%的正常子宫内膜可见UEA-1结合，而60%的EAC可见H抗原，正常子宫内膜中H抗原表达率为0%。在腺癌中，不仅在腺管腔缘可见染色，在细胞质以及细胞外侧或基底细胞膜也可见染色，而在正常子宫内膜中，染色大多位于腔缘。因此，在肿瘤性和非肿瘤性子宫内膜中观察到糖缀合物的阳性率和定位存在差异。
